TRB-3 Antibody (D-4)recommended for detection of TRB-3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

TRB-3 Antibody (D-4): sc-365842

TRB-3 Antibody (D-4) is rated 3.8 out of 5 by 8.
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Datasheets

    • TRB-3 Antibody (D-4) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-165 mapping at the N-terminus of TRB-3 of mouse origin
    • TRB-3 Antibody (D-4) is recommended for detection of TRB-3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • TRB-3 Antibody (D-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of TRB-3 (D-4): sc-365842.
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I am using TRB-3 (D-4): sc-365842 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Germaine
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from Not work Use Tm(ER stress-inducer, 5ug/ml)-treated hepa1-6 cell samples, dilution to 1:500, not see any band in the right protein size
Date published: 2017-08-17
Rated 5 out of 5 by from perform in the wb asked the sample for the preliminary experiment,and the result is nice.
Date published: 2017-08-13
Rated 5 out of 5 by from The function of this TRB3 antibody is excellent I have bought the product a month ago, and the experiment results turned out to be very good. We were pleased to see the function of TRB3 antibody was stable and the price was very acceptable.
Date published: 2017-08-09
Rated 5 out of 5 by from Antibody performed very well in Western Blot This is the first time experience with TRB-3 and was a sample test from Santa Cruz. This marker was used in Human muscle and adipose tissue that is part of a Diabetes study in conjunction with the TXNIP pathway. The antibody performed very well in a Western Blot application and yielded a product size of 45kDa as indicated by the company. One band was present with a fairly clean signal.
Date published: 2017-05-11
Rated 1 out of 5 by from I am sorry that the product I used did not work. S-D rat ,pulmonary artery smooth muscle cell. the product I applied for western blot test did not work.I used 1:100 and tried twice ,did not get the result.
Date published: 2016-12-27
Rated 4 out of 5 by from Good band seen in WB using Hep G2 whole cell Good band seen in WB using Hep G2 whole cell lysate. -SCBT QC
Date published: 2015-06-28
Rated 5 out of 5 by from Produced great immunoperoxidase nuclear staining Produced great immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human pancreas tissue. -SCBT QC
Date published: 2015-04-29
Rated 4 out of 5 by from Worked well with transfected lysate by WB Worked well with transfected lysate by WB. -SCBT QC
Date published: 2015-03-07
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