TRAP95 Antibody (F83): sc-130439

4.0(4)

Write a review Ask a question

Datasheets

- TRAP95 Antibody (F83) is a mouse monoclonal IgG_2b κ TRAP95 antibody, cited in 1 publications, provided at 100 µg/ml
- raised against recombinant TRAP95 of human origin
- recommended for detection of TRAP95 of human origin by WB, IP and IF
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of TRAP95 (F83): sc-130439.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for TRAP95 Antibody (F83). This work is in progress.

SEE ALSO...

Additional Thyroid Hormone Receptor Associated Protein Antibodies including TRAP150, TRAP220, TRAP230 and TRAP240
Learn more about our ImmunoCruz® Antibody Conjugates and Cruz Marker™ MW Standards Promotional

TRAP95 Antibody (F83) - Western Blotting - Image 54004

TRAP95 Antibody (F83) - Immunofluorescence - Image 53939

TRAP95 Antibody (F83) - Western Blotting - Image 65775

Click on image or enlarge button to enlarge

TRAP95 Antibody (F83) is an IgG_2b κ mouse monoclonal TRAP95 antibody (also designated TRAP95 antibody) suitable for the detection of the TRAP95 protein of human origin by WB, IP and IF. TRAP95 Antibody (F83) is available as the non-conjugated anti-TRAP95 antibody. In mammalian cells, transcription is regulated in part by high molecular weight coactivating complexes that mediate signaling between transcriptional activators and initiation factors. These complexes include the thyroid hormone receptor-associated protein (TRAP) complex, which interacts with thyroid receptors (TR), vitamin D receptors and other steroid receptors to facilitate hormone induced transcriptional activation. The TRAP complex consists of numerous proteins ranging in size including TRAP95, TRAP100, TRAP150, TRAP220 and TRAP230, that are characterized by the presence of a nuclear receptor recognition motif which mediates the ligand-dependent binding of TRAP proteins to the nuclear receptors. TRAP220 and TRAP100 are widely expressed and most abundantly detected in skeletal muscle, heart and placenta. TRAP95, TRAP150 and TRAP230 facilitate TR induced transcription by associating with an additional transcriptional coactivating complex SMCC (SRB and MED protein cofactor complex), which consists of various subunits that share homology with several components of the yeast transcriptional mediator complexes.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

A novel human SRB/MED-containing cofactor complex, SMCC, involved in transcription regulation. | Gu, W., et al. 1999. Mol Cell. 3: 97-108. PMID: 10024883
Competition between thyroid hormone receptor-associated protein (TRAP) 220 and transcriptional intermediary factor (TIF) 2 for binding to nuclear receptors. Implications for the recruitment of TRAP and p160 coactivator complexes. | Treuter, E., et al. 1999. J Biol Chem. 274: 6667-77. PMID: 10037764
Identity between TRAP and SMCC complexes indicates novel pathways for the function of nuclear receptors and diverse mammalian activators. | Ito, M., et al. 1999. Mol Cell. 3: 361-70. PMID: 10198638
Identification of mouse TRAP100: a transcriptional coregulatory factor for thyroid hormone and vitamin D receptors. | Zhang, J. and Fondell, JD. 1999. Mol Endocrinol. 13: 1130-40. PMID: 10406464
The structure of the nuclear hormone receptors. | Kumar, R. and Thompson, EB. 1999. Steroids. 64: 310-9. PMID: 10406480
The TRAP220 component of a thyroid hormone receptor- associated protein (TRAP) coactivator complex interacts directly with nuclear receptors in a ligand-dependent fashion. | Yuan, CX., et al. 1998. Proc Natl Acad Sci U S A. 95: 7939-44. PMID: 9653119
Mammalian mediator of transcriptional regulation and its possible role as an end-point of signal transduction pathways. | Jiang, YW., et al. 1998. Proc Natl Acad Sci U S A. 95: 8538-43. PMID: 9671713

Species	Gene Name	Gene ID	Chromosome Location	Isoform (mRNA) Accession Number	Protein Accession Number	OMIM™ Number
Human	MED16	Entrez Gene ID: 10025	19p13.3	XM_017026120, XM_017026121, NM_005481	UniProt ID: Q9Y2X0	604062

Download Datasheet

Protocols Online

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TRAP95 Antibody (F83) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-TRAP95 Antibody (F83) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TRAP95 Antibody (F83) Specifications

Alternate Product Name

TRAP95

Product Clone Name

F83

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG_2b kappa light chain

Amino Acids

1-111

Epitope Target

TRAP95

Epitope Species

human

Product Reactivity/Detection

TRAP95

Species Reactivity/Detection

human

Additional Mammalian Reactivity

N/A

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF

Vialing Information

Each vial contains 100 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

1 citations in various scientific publications

Recommended Secondary Detection Reagents

N/A

Available Conjugates/Forms

N/A

Primary antibodies for non-mammalian targets are useful in detecting proteins of plant, viral, bacterial, avian, fish, yeast and invertebrates. Some examples include Drosophila, zebrafish, Xenopus, Arabidopsis, C. elegans, Clostridium, Campylobacter, Heliobacter pylori, Hepatitis, HIV, HPV, HSV, Influenza, Klebsiella, M. tuberculosis, Plasmodium, Pseudomonas, RSV, Saccharomyces cerevisiae, Salmonella, Schistosoma, Shigella and Staphylococcus.

Santa Cruz Biotechnology monoclonal antibodies can be used in assays including flow cytometry, immunoprecipitation, Western blot analysis, and immunostaining. Anti-TRAP95 Antibody (F83) is recommended for use in the following applications: WB (Western blotting), IP (Immunoprecipitation), IF (Immunofluorescence).

Mouse secondary antibodies, control sera and control immunoglobulins are also offered to be used in combination with our primary monoclonal antibodies.

Tissue extracts, nuclear extracts and whole cell lysates are used with Santa Cruz Biotechnology monoclonal antibodies for western blotting or immunoprecipitation positive controls. Nuclear extracts are ideal for western blot analysis or gel shift assays positive controls. Phospho-enriched whole cell lysates and tissue extracts are available for phospho-specific antibody positive controls.

Monoclonal secondary antibodies are offered in various conjugated forms, including: Biotin (B), CruzFluor™ 790, 680, 647, 594, 555, and 488, FITC, HRP, PerCP, PerCP-Cy5.5, Texas Red (TR), and TRITC (tetramethyl rhodamine isothiocyanate) (R), for research applications like Flow Cytometry (FCM), Immunofluorescence (IF), Immunohistochemistry (IHC) staining and Western Blotting (WB). Not intended for diagnostic or therapeutic use.

Affinity purified immunoglobulins (IgGs)—conjugated and unconjugated—are available as negative controls for flow cytometry (FCM), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP) and Western Blotting (WB) applications.

Normal sera for immunohistochemistry (IHC) applications are available as neat serum for blocking reagents. The serum which is used should match the species of the secondary antibody.

I am using TRAP95 (F83): sc-130439 for immunofluorescence, what dilution of this antibody should I use?

Asked by: Cweed

Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining

Answered by: Technical Support

Date published: 2017-03-24

Rated 4 out of 5 by JT from Positive results in WB using human TRAP95 Positive results in WB using human TRAP95 transfected 293T whole cell lysates and HeLa nuclear extract. -SCBT QC

Date published: 2015-06-27

Rated 4 out of 5 by FL from Published western blot data using human samples Published western blot data using human samples. -SCBT Publication Review

Date published: 2015-04-18

Rated 4 out of 5 by LF from Clean band in western blot analysis of TRAP95 Clean band in western blot analysis of TRAP95 expression in HeLa nuclear extract. -SCBT QC

Date published: 2015-04-03

Rated 4 out of 5 by MS from Robust immunofluorescence nuclear staining Robust immunofluorescence nuclear staining in methanol-fixed HeLa cells. -SCBT QC

Date published: 2014-11-02

Choose a Store

Choose a Language

Choose a Currency

TRAP95 Antibody (F83): sc-130439

QUICK LINKS

SEE ALSO...

References:

TRAP95 Antibody (F83) Specifications

Alternate Product Name

Product Clone Name

Classification

Clonality

Antibody Isotype

Amino Acids

Epitope Target

Epitope Species

Product Reactivity/Detection

Species Reactivity/Detection

Additional Mammalian Reactivity

Additional Non-Mammalian Reactivity

Applications

Vialing Information

Research Citations

Recommended Secondary Detection Reagents

Available Conjugates/Forms

I am using TRAP95 (F83): sc-130439 for immunofluorescence, what dilution of this antibody should I use?