TRAF6 Antibody (D-10)recommended for detection of TRAF6 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

TRAF6 Antibody (D-10): sc-8409

TRAF6 Antibody (D-10) is rated 4.7 out of 5 by 21.
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Datasheets

    • TRAF6 Antibody (D-10) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-274 mapping at the N-terminus of TRAF6 of human origin
    • TRAF6 Antibody (D-10) is recommended for detection of TRAF6 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • TRAF6 Antibody (D-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of TRAF6 (D-10): sc-8409.
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Dear all, I would like to test your traf 6 (D10) antibody (sc8409) by Western blot. Would it be possible to send me a small aliquot? Thanks is advance, kind regards Chantal

Asked by: Anonymous
Thank you for your question. Please contact Technical Service or your local distributor for information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Tech Service
Date published: 2019-09-30

Hello. I am a PhD student and analysing expression in inflammatory pathways. I was wondering if I could get a free sample of 10 µg sample of TRAF6 (D-10): sc-8409. Thank you in advance.

Asked by: Denise Carrasco
Thank you for your question. If you have an interest in testing a free 10 µg sample of sc-8409, please contact our Technical Service by phone or email or by Live Chat.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-21

Hi, I am part of an innate immunology research laboratory and am currently in the process of obtaining an anti-TRAF6 antibody for upcoming WB, IP and IF assays. Is it possible to receive the 10ug free sample advertised above for TRAF6 (D-10): sc-8409?

Asked by: Keaton
Thank you for your question. Please contact your local distributor in Australia, they will be happy to send you any samples you wish to test. You can find their contact information here: https://www.scbt.com/scbt/resources/distributors
Answered by: Tech Service
Date published: 2017-08-14

What is a suitable positive control cell line for testing the TRAF6 Antibody (D-10): sc-8409 in Western blot?

Asked by: cjMara
Thank you for your question. We recommend using either HeLa (sc-2200) or WEHI-3 (sc-3815) whole cell lysates for Western blot testing with this TRAF6 antibody.
Answered by: Technical Support
Date published: 2016-12-21

What is the exact epitope recognized by TRAF6 (D-10): sc-8409?

Asked by: cjMara
Thank you for your question. The (D-10) clone is raised against amino acids 1-274 mapping at the N-terminus of TRAF6 of human origin. The exact epitope recognized by this mouse monoclonal antibody has not been determined.
Answered by: Technical Support
Date published: 2016-12-21
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Rated 4 out of 5 by from Good signal on WB I got a good signal on WB (1:200 in 5% milk), although there were some non-specific bands.
Date published: 2018-10-16
Rated 4 out of 5 by from TRAF6 Antibody (D-10) SAMPLE Review Mouse anti-TRAF6 antibody (sc-8406) SAMPLE works well in WB at 1:1000 in 5 % BSA with LN229 cells and can be reused. The background of bands were clean.
Date published: 2017-12-27
Rated 5 out of 5 by from TRAF6试用装抗体使用体会 大概三个月前,申请了试用装抗体,发货很快,工作人员也很热情,我做石蜡切片的免疫组化,发现并没有我想要的阳性染色,仔细查文献后发现结果应该如此,感谢santa cruz,使我能够及时调整课题。
Date published: 2017-12-04
Rated 4 out of 5 by from Spécifique Testé sur 13 µg de protéines extraites de cellules COV434. L'anticorps a été dilué au 1/200 en TBS-Tween + 5% lait écrémé. Une faible bande est détectée au poids moléculaire attendu après 30 min d'exposition. Pas de bandes aspécifiques.
Date published: 2017-10-11
Rated 5 out of 5 by from IP TRAF6 IP TRAF6 in macrophage, 1ug antibody in 1ml lysate, 4 degrees centigrade,overnight, but did not get right band.
Date published: 2017-10-10
Rated 4 out of 5 by from Works with a porcine cell line Works with porcine macrophage for immunofluorescence.
Date published: 2017-09-30
Rated 5 out of 5 by from Anti-TRAF6 antibody Mouse anti-TRAF6 antibody (sc-8406) works great on immunoblotting at 1:1000 in 2.5 % milk powder and can be reused. It works well on IP at 1 ug for 1 ml of cell lysate or 300 ug of tissue lysate.
Date published: 2017-08-24
Rated 4 out of 5 by from I'd Use It Again TRAF6 Ab (D-10) did the job, good sensitivity and results.
Date published: 2017-08-10
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