Anti-TR2 Antibody (D-4) recommended for detection of TR2 of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-TR2 Antibody (D-4): sc-374049

Anti-TR2 Antibody (D-4) is rated 5.0 out of 5 by 3.
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Datasheets

    • Anti-TR2 Antibody (D-4) is a mouse monoclonal IgG1 (kappa light chain) TR2 antibody provided at 200 µg/ml
    • raised against amino acids 25-110 of TR2 of mouse origin
    • Anti-TR2 Antibody (D-4) is recommended for detection of TR2 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-TR2 Antibody (D-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of TR2 (D-4): sc-374049.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for TR2 Antibody (D-4) for WB applications. These reagents are now offered in bundles with TR2 Antibody (D-4) (see ordering information below).
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What is the recommended fixation method for immunofluorescence staining with TR2 (D-4): sc-374049 monoclonal antibody?

Asked by: jerojero
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from 美しいバンドが検出されます。 WBで使用しました。シャープなバンドが検出されました。非特異的なバンドは一切ありません。バンドの大きさもデータシートと同じ位置です。
Date published: 2020-12-01
Rated 5 out of 5 by from Excellent immunofluorescence nuclear staining Excellent immunofluorescence nuclear staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-02-11
Rated 5 out of 5 by from Great Western Blot data of TR2 expression Great Western Blot data of TR2 expression in HeLa whole cell lysate. -SCBT QC
Date published: 2014-01-11
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