Toxin B, isolated from Clostridium difficile, is a high molecular weight glucosyltransferase that inhibits small GTPases, such as Rho, Rac, and Cdc42, via glucosylation of a threonine residue. This results in the shutdown of signal transduction cascades, which leads to depolymerization of the cytoskeleton, gene transcription of a number of stress-activated protein kinases (SAPKs), and a decrease in the synthesis of phosphatidylinositiol 4,5-bisphosphate. Toxin B may also block serotonin release and stimulate the production of phospholipases C and D. Due to its ability to affect depolymerization of the cytoskeleton, toxin B has been shown to inhibit biphasic muscle contraction. This toxin does not require assistance to permeabilize cells, unlike exoenzyme C3. It is cytotoxic as determined in a cell rounding assay using human foreskin cells. When reconstituted with 100 uL of H2O each vial will contain 2 ug of C. difficile toxin B dissolved in 50 mM Tris, pH 7.5, 100 mM NaCl, with 0.1% trehalose
1. Prepens, U., et al., 1996. Inhibition of Fc epsilon-RI-mediated activation of rat basophilic leukemia cells by Clostridium difficile toxin B (monoglucosyltransferase) The Journal of biological chemistry. 271(13): 7324-9. PMID: 8631752
2. Wilkins, T D., et al., 1996. Clostridium difficile toxins attack Rho. Trends in microbiology. 4(2): 49-51. PMID: 8820565
3. Schmidt, M., et al., 1996. Restoration of Clostridium difficile toxin-B-inhibited phospholipase D by phosphatidylinositol 4,5-bisphosphate. European journal of biochemistry / FEBS. 240(3): 707-12. PMID: 8856074
4. Mackay, D J., et al., 1998. Rho GTPases. The Journal of biological chemistry. 273(33): 20685-8. PMID: 9694808
Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Call us Toll Free at 1-800-457-3801.
Copyright © 2007-2019 Santa Cruz Biotechnology, Inc. All Rights Reserved. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.
All trademarks are the property of their respective owners.