Tom22 Antibody (J-31)recommended for detection of Tom22 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Tom22 Antibody (J-31): sc-101286

Tom22 Antibody (J-31) is rated 4.2 out of 5 by 6.
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Datasheets

    • Tom22 Antibody (J-31) is a mouse monoclonal IgG2a (kappa light chain) provided at 100 µg/ml
    • raised against recombinant Tom22 of human origin
    • recommended for detection of Tom22 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Tom22 (J-31): sc-101286.
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What is the recommended secondary reagent to use with Tom22 (J-31): sc-101286 for Western blot when working with human samples?

Asked by: Trav11
Thank you for your question. We recommend using one of our exclusive Mouse IgG Binding Proteins as a secondary detection reagent, such as m-IgGκ BP-HRP: sc-516102, for Western blot. A complete list of all available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-8301 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Technical Support 7
Date published: 2017-01-24
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Rated 2 out of 5 by from no crossreactivity in C. elegans detected we wanted to test whether this antibody against human TOM22 might crossreact with the ortholog in the nematode C. elegans. Unfortunately, we did not detect a respective band in western blot analysis of C. elegans protein lysate.
Date published: 2018-04-27
Rated 5 out of 5 by from Great product Strong data for western blot, IF and IHC staining applications
Date published: 2017-02-05
Rated 5 out of 5 by from Really strong antibody! This Tom22 antibody, sc-101286, is really reliable and gives nice solid bands in Western blot. IHC staining was nice and specific as well, so a well rounded antibody, for sure.
Date published: 2017-01-11
Rated 5 out of 5 by from Reproducable Reproducable, single band observed in WB with NIH/3T3 whole cell lysates -SCBT QC
Date published: 2015-04-22
Rated 4 out of 5 by from Good cytoplasmic staining observed in IHC Good cytoplasmic staining observed in IHC with paraffin-embedded, formalin fixed human small intestine issue -SCBT QC
Date published: 2015-02-27
Rated 4 out of 5 by from Works well in IF with paraformaldehyde Works well in IF with paraformaldehyde-fixed HeLa cells -SCBT QC
Date published: 2014-04-18
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