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The mitochondrial preprotein translocases of the outer membrane (Tom) is a multisubunit protein complex that facilitates the import of nucleus-encoded precursor proteins across the mitochondrial outer membrane. The Tom machinery consists of import receptors for the initial binding of cytosolically synthesized preproteins and a general import pore (GIP) for the membrane translocation of various preproteins into the mitochondria. The import receptors include Tom20 and Tom22, which form a heteromeric receptor complex that initiates the insertion of newly synthesized proteins into the outer membrane and then directs the precursor protein into the GIP. In yeast, Tom22 is the essential component of the import receptor complex, as it functions as a receptor for the preproteins and serves as a docking point for both Tom20 and the GIP (5,6). Tom22 directly associates with Tom40, the major component of the GIP, and forms a stable interaction between the two core complexes. This interactions facilitates the fluid movement of preproteins into the mitochondria. Structural features of Tom22 include an N-terminal negatively charged region exposed to the cytosol, a C-terminal innermembrane space region with little negative charge, and a putative transmembrane region. The gene encoding human Tom22 maps to chromosome 22q12-q13 (9,10).
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
What is the recommended secondary reagent to use with Tom22 (J-31): sc-101286 for Western blot when working with human samples?
Asked by: Trav11
Thank you for your question. We recommend using one of our exclusive Mouse IgG Binding Proteins as a secondary detection reagent, such as m-IgGκ BP-HRP: sc-516102, for Western blot. A complete list of all available binding proteins is available on our website here:
If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-8301 option 2, emailing email@example.com, or using the Live Chat function on our website.
Rated 2 out of
no crossreactivity in C. elegans detectedwe wanted to test whether this antibody against human TOM22 might crossreact with the ortholog in the nematode C. elegans. Unfortunately, we did not detect a respective band in western blot analysis of C. elegans protein lysate.
Date published: 2018-04-27
Rated 5 out of
Great productStrong data for western blot, IF and IHC staining applications
Date published: 2017-02-05
Rated 5 out of
Really strong antibody!This Tom22 antibody, sc-101286, is really reliable and gives nice solid bands in Western blot. IHC staining was nice and specific as well, so a well rounded antibody, for sure.
Date published: 2017-01-11
Rated 5 out of
ReproducableReproducable, single band observed in WB with NIH/3T3 whole cell lysates -SCBT QC
Date published: 2015-04-22
Rated 4 out of
Good cytoplasmic staining observed in IHCGood cytoplasmic staining observed in IHC with paraffin-embedded, formalin fixed human small intestine issue -SCBT QC
Date published: 2015-02-27
Rated 4 out of
Works well in IF with paraformaldehydeWorks well in IF with paraformaldehyde-fixed HeLa cells -SCBT QC
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