Tom1 Antibody (H-5)recommended for detection of Tom1 of mouse, rat and human origin by WB, IP, IF and ELISA

Tom1 Antibody (H-5): sc-514430

Tom1 Antibody (H-5) is rated 4.0 out of 5 by 3.
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Datasheets

    • Tom1 Antibody (H-5) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 368-439 mapping near the C-terminus of Tom1 of human origin
    • Tom1 Antibody (H-5) is recommended for detection of Tom1 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Tom1 Antibody (H-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Tom1 (H-5): sc-514430.
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For Western Blot, is it recommended to use denatured or non-denatured conditions with Tom1 (H-5): sc-514430 monoclonal antibody?

Asked by: AbPolly
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Excellent antibody I am strongly recommended this antibody for mitochondrial staining. It's work against human monocyte derived macrophages mitochondria but didn't work on mouse bone marrow derived macrophages mitochondria.
Date published: 2017-09-25
Rated 3 out of 5 by from Signal in WB, not in IF Good signal in WB on mouse macrophages, 1/150. No signal in IF on PFA-fixed mouse neurons 1/100.
Date published: 2017-08-24
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