Anti-TNF alpha Antibody (TN3-19.12) recommended for detection of TNFα of mouse, rat and rabbit origin by WB, IP and IF

Anti-TNFα Antibody (TN3-19.12): sc-12744

Anti-TNFα Antibody (TN3-19.12) is rated 4.2 out of 5 by 19.
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Datasheets

    • Anti-TNFα Antibody (TN3-19.12) is a Armenian hamster monoclonal IgG TNFα antibody, cited in 28 publications, provided at 200 µg/ml
    • raised against purified recombinant TNFα of mouse origin
    • Anti-TNF alpha Antibody (TN3-19.12) is recommended for detection of TNFα of mouse, rat and rabbit origin by WB, IP and IF
    • Anti-TNF alpha Antibody (TN3-19.12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also provided azide-free for neutralization, sc-12744 L, 200 µg/0.1 ml
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of TNFα (TN3-19.12): sc-12744.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for TNFα Antibody (TN3-19.12). This work is in progress.
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A manuscript reviewer requested we provide validation for this antibody. Do you have any internal data for IF?

Asked by: bawatts
Thank you for your question. We do not currently have IF data available for this antibody. The most current source of data for our antibodies is our website, so please check back for updates! There are multiple product citations using this antibody for IF or IHC applications. These include PMID's: 30911021 and 27681360.
Answered by: Technical Support
Date published: 2020-05-05

Can I use horse-anti mouse secondary antibody on this product (hamster) as the DAB kit we're using does not provide horse-anti hamster secondary antibody

Asked by: mnopqr
Thank you for your question. This is an Armenian hamster monoclonal IgG, and we recommend using anti-Armenian hamster IgG secondary antibody for detection. We do not know if anti-mouse IgG secondary antibody would be suitable for detection.
Answered by: Technical Support
Date published: 2019-05-13

can i get two band using this antibody , and i didnot get a clear band between 15-25, have you ever get two bands ?

Asked by: jiapangpang1993
Thank you for your product review/feedback. We are sorry to hear that this antibody did not perform for you as expected. We want you to be successful in your research. Please contact our Technical Service Department by phone, e-mail or live chat. We are happy to help you troubleshoot the antibody. Santa Cruz Biotechnology does warranty our antibodies for one year from the date of purchase. If we are unable to successfully troubleshoot your application we will send you a replacement antibody free of charge. Please contact your corresponding Technical Service department: https://www.scbt.com/scbt/resources/technical-service
Answered by: Technical Support Europe
Date published: 2017-10-23

Can TNFα (TN3-19.12): sc-12744 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: TinTin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, TNFα (TN3-19.12): sc-12744 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from good antibody antibody was used in 1:500 dilution in mice liver lysates. Results were in general good, the tricky point will be the secondary antibody.
Date published: 2018-09-05
Rated 5 out of 5 by from Great feuture Excellent specificity! very good bands for western blot.
Date published: 2017-11-03
Rated 4 out of 5 by from Great antibody I used it for western blotting at 1:1000 dilution. The signal was great.
Date published: 2017-10-26
Rated 4 out of 5 by from Good antibody Worked well with western blotting at 1:500 dilution.
Date published: 2017-09-27
Rated 3 out of 5 by from 抗体效果一般 年初,申请了该抗体的单克隆试用装,效果一般。用的组织标本做的WB,条带微弱,可能本身表达量不高,上样量需要加倍。
Date published: 2017-09-25
Rated 5 out of 5 by from TNF效果评价 大概八月份的时候,和其他的几个基因一起进行申请,效果还不错,值得去使用和购买,毕竟是大公司的产品,质量有保证
Date published: 2017-09-20
Rated 3 out of 5 by from 一般 用于人脐静脉内皮细胞来源的蛋白进行WB检测,背景比较深,条带弱,不够清晰,可能后面还需要再优化下条件
Date published: 2017-08-07
Rated 5 out of 5 by from Works okay on flow cytometry Works okay on flow cytometry on mouse cell line compared to isotype control.
Date published: 2017-07-14
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