Anti-TNF alpha Antibody (J1D9) recommended for detection of TNFα of mouse, rat and human origin by WB, IP and FCM

Anti-TNFα Antibody (J1D9): sc-33639

Anti-TNFα Antibody (J1D9) is rated 4.0 out of 5 by 1.
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Datasheets

    • TNFα Antibody (J1D9) is a mouse monoclonal IgG1 κ, cited in 7 publications, provided at 200 µg/ml
    • raised against purified recombinant human TNFα
    • Anti-TNF alpha Antibody (J1D9) is recommended for detection of TNFα of mouse, rat and human origin by WB, IP and FCM
    • Anti-TNF alpha Antibody (J1D9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also provided azide-free for neutralization, sc-33639 L, 200 µg/0.1 ml
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of TNFα (J1D9): sc-33639.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for TNFα Antibody (J1D9) for WB applications. These reagents are now offered in bundles with TNFα Antibody (J1D9) (see ordering information below).
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thanks for your answer but can you please mention incubation time for tnf alpha hrp conjugated antibody?

Asked by: manoj
Thank you for your question. We recommend incubating with Anti-TNFα Antibody (J1D9): sc-33639 HRP ar +4 °C over night.
Answered by: Tech Support Europe
Date published: 2021-04-14

can you please elaborate the application of SC-33639 HRP antibody?

Asked by: manoj
Thank you for your question. The HRP-conjugated version of Anti-TNFα Antibody (J1D9): sc-33639 can be used for WB, IHC(P) and ELISA and does not require a secondary detection antibody.
Answered by: Tech Support Europe
Date published: 2021-04-09

Can TNFα (J1D9): sc-33639 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Professor Griffin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, TNFα (J1D9): sc-33639 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Good band achieved in Western blot Good band achieved in Western blot.
Date published: 2013-02-07
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