Anti-TNF alpha Antibody (C-4) recommended for detection of TNFα of and human origin by WB, IP, IF and ELISA

Anti-TNFα Antibody (C-4): sc-133192

Anti-TNFα Antibody (C-4) is rated 4.1 out of 5 by 17.
  • y_2021, m_7, d_26, h_19
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.18
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_17
  • loc_en_US, sid_133192, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 90ms
  • REVIEWS, PRODUCT
loading
5
8
4
4
3
4
2
1
1
0

Datasheets

    • TNFα Antibody (C-4) is a mouse monoclonal IgG2a κ, cited in 30 publications, provided at 200 µg/ml
    • raised against amino acids 77-233 of TNFα of human origin
    • TNF alpha Antibody (C-4) is recommended for detection of TNFα of human origin by WB, IP, IF and ELISA
    • Anti-TNF alpha Antibody (C-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of TNFα (C-4): sc-133192.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for TNFα Antibody (C-4) for WB applications. These reagents are now offered in bundles with TNFα Antibody (C-4) (see ordering information below).
loading

Is a blocking peptide for this product available?

Asked by: dragonfly
Thank you for your question. This mouse monoclonal antibody, TNFα (C-4): sc-133192 is not raised against a peptide so no blocking peptide is available. The antibody is raised against amino acids 77-233 of TNFα of human origin.
Answered by: Technical Support
Date published: 2017-09-27

What is the recommended starting dilution for Western blot for TNFα (C-4): sc-133192?

Asked by: jenni
Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-133192.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support 8
Date published: 2018-01-21
  • y_2021, m_7, d_27, h_19CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.18
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_en_US, sid_133192, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 112ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from good for WB I can detect tnfa by WB. I used macrophage (RAW264.7) and macrophage CM.
Date published: 2018-07-13
Rated 2 out of 5 by from Didn't detect any TNF in my blots I am not sure if the antibody worked or not because I was not able to detect any TNF in my western blots and did not run a positive control.
Date published: 2018-03-13
Rated 4 out of 5 by from sc-133192 We used it to detect brain samples. A clear band at the correct size. There are two more prominent bands at other sizes, too
Date published: 2017-10-03
Rated 4 out of 5 by from Good antibody Worked well with western blotting at 1:500 dilution.
Date published: 2017-09-27
Rated 4 out of 5 by from sc-133192 we use it on Caco cells using WB,. Work well.
Date published: 2017-08-21
Rated 3 out of 5 by from NOT VERY GOOD TNF-A is not good to IHC,but other use not to do it .
Date published: 2017-07-21
Rated 3 out of 5 by from band is fuzzy the antibody samples arrived soon, but the protein band is fuzzy
Date published: 2017-07-18
Rated 3 out of 5 by from applicate it into IHC we've applicated this antibody into the experiment of IHC , but the result is not we want because we got the positive results but in the published papers they are the negative results
Date published: 2017-05-31
  • y_2021, m_7, d_26, h_19
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.18
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_17
  • loc_en_US, sid_133192, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 9ms
  • REVIEWS, PRODUCT