Anti-TNF alpha Antibody (52B83) recommended for detection of natural and recombinant TNFα of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA

Anti-TNFα Antibody (52B83): sc-52746

Anti-TNFα Antibody (52B83) is rated 4.3 out of 5 by 22.
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Datasheets

    • TNFα Antibody (52B83) is a mouse monoclonal IgG1 κ, cited in 317 publications, provided at 100 µg/ml
    • raised against purified full length native TNFα of human origin
    • recommended for detection of natural and recombinant TNFα of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA; also reactive with additional species, including and Guinea pig, Chimpanzee, Cynomolgus and Rhesus monkey; non cross-reactive with TNFβ or lymphotoxin
    • See TNFα (C-4): sc-133192 for TNFα antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of TNFα (52B83): sc-52746.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for TNFα Antibody (52B83) for WB and IHC(P) applications. These reagents are now offered in bundles with TNFα Antibody (52B83) (see ordering information below).
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I would like to have a sample from two TNFa antibodies TNFa (C-4) sc133192 and TNFa-(52B83) sc-52746 to be able to decide which to order for my further experiments. Thank you in advance, T.B.

Asked by: Tzvete
Thank you for your question. Please contact Technical Service for information on how to receive free 10 ug samples of these monoclonal antibodies.
Answered by: Tech Service
Date published: 2019-07-10

Is this antibody knockout verified?

Asked by: UmNj
Thank you for your question. We have not performed any RNAi or CRISPR knockout or knockdown experiments to verify the specificity of this specific mouse monoclonal, TNFα Antibody (52B83): sc-52746. This particular antibody is very popular, and a well established clone that has been featured in over 50 publications, which are available on our website.
Answered by: Technical Support
Date published: 2017-03-16

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: Sweetums
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 4
Date published: 2016-12-16

I need to use an antibody to detect the protein in samples of (other species, e.g., bovine, porcine, canine, equine, avian, non-mammalian) origin. Do you know if this mouse monoclonal antibody will work?

Asked by: Sweetums
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Tech Service 4
Date published: 2016-12-16
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Rated 1 out of 5 by from Nessun segnale in WB Testato su campioni di linea murina di macrofagi RAW264.7 stimolati con 10 o 100 ng/mL di LPS per 4 o 24 ore. L'anticorpo di un altro produttore ha funzionato.
Date published: 2021-04-23
Rated 5 out of 5 by from Good Western Blot results We obtained a strong signal using this antibody in western blot. Band on predicted kDa height.
Date published: 2021-01-28
Rated 4 out of 5 by from Bene sia in immunofluoirescenza che in wb Ho provato questo anticorpo in immunofluoresenza (1:100) su spinal cord di topo (fissato e criostatato), sia in wb (1:1000) e in entambi i casi ha funzionato molto bene
Date published: 2020-10-05
Rated 4 out of 5 by from Western Blo We obtained a strong signal using this antibody in western blot. Band on predicted kDa height.
Date published: 2019-10-07
Rated 4 out of 5 by from 使用,无杂带 本实验室于一年前购买了MHC-II的抗体,满足实验需求,条带明显且无杂带,等到了让人欣喜的实验结果。
Date published: 2019-05-16
Rated 5 out of 5 by from WB working well this sc-52746 TNFa antibody workes well on WB, gives nice bands
Date published: 2019-04-16
Rated 4 out of 5 by from It's good for WB! I don't know the exactly time we bought this antibody, but I think it was bought at least three years ago. But it's still good for WB at 1:500.
Date published: 2018-10-29
Rated 5 out of 5 by from Great product I bought this month TNFalpha antibody and I run western blotting analysis on total cell extract of Ehrlich Ascites Carcinoma. The results were wonderful and the antibody worked well in detecting the difference in protein level between the experimental groups.
Date published: 2018-08-09
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