TNFβ Antibody (359-81-11) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
raised against recombinant TNFβ of human origin
recommended for detection of TNFβ of human origin by IP and FCM
available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
also provided azide-free for neutralizing, sc-32767 L, 200 µg/0.1 ml
m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for TNFβ Antibody (359-81-11) for WB and IHC(P) applications. These reagents are now offered in bundles with TNFβ Antibody (359-81-11) (see ordering information below).
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Tumor necrosis factor b (TNFb), also known as lymphotoxin, is a pleiotropic cytokine. TNFa, also known as cachectin, is a smaller cytokine that binds to the same receptors, producing a vast array of effects similar to those of TNFb. TNFb and TNFa share 30% amino acid homology and have similar biological activities. TNFb is produced by activated lymphocytes, including CD4(+) T helper cell type 1 lymphocytes, CD8(+) lymphocytes and certain B lymphoblastoid cell lines. TNFa is produced by several different cell types, including lymphocytes, neutrophils and macrophages. TNFa and TNFb can modulate many immune and inflammatory functions, while having the ability to inhibit tumor growth. Target tumor cells must express TNF receptors 1 and 2 to be killed, with the p55 receptor mediating the cytotoxic response.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Preciso deste anticorpo não conjugado para ser aplicado em tecidos humanos parafinados para imunohistoquimica com revelação por DAB.
Asked by: Maria dos Anjos
Thank you for your question. We do not have any data or product citations using this antibody for immunohistochemistry in paraffin-embedded tissue sections and so IHC(P) is not a recommended application at this time.
Answered by: Technical Support
Date published: 2019-09-11
How can TNFβ (359-81-11): sc-32767 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?
Asked by: AbPolly
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to phycoerythrin (sc-32767 PE) or fluoroscein (sc-32767 FITC).
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