Anti-TNF alpha Antibody (E-4) recommended for detection of TNFα of human origin by WB, IP, IF and ELISA

Anti-TNFα Antibody (E-4): sc-133193

Anti-TNFα Antibody (E-4) is rated 4.5 out of 5 by 2.
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Datasheets

    • TNFα Antibody (E-4) is a mouse monoclonal IgG2a κ, cited in 4 publications, provided at 200 µg/ml
    • raised against amino acids 77-233 of TNFα of human origin
    • recommended for detection of TNFα of human origin by WB, IP, IF and ELISA
    • See TNFα (C-4): sc-133192 for TNFα antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for TNFα Antibody (E-4) for WB applications. These reagents are now offered in bundles with TNFα Antibody (E-4) (see ordering information below).
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What is the recommended fixation method for immunofluorescence staining with TNFα (E-4): sc-133193 monoclonal antibody?

Asked by: jenniferc
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 4 out of 5 by from Positive signal at the correct molecular Positive signal at the correct molecular weight in Western Blot. -SCBT QC
Date published: 2015-03-24
Rated 5 out of 5 by from Produced superb immunofluorescence cytoplasmic Produced superb immunofluorescence cytoplasmic and membrane staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-02-16
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