Anti-TNF alpha Antibody (AS1) recommended for detection of TNFα of human origin by WB, IP and ELISA

Anti-TNFα Antibody (AS1): sc-52791

Anti-TNFα Antibody (AS1) is rated 4.0 out of 5 by 1.
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Datasheets

    • TNFα Antibody (AS1) is a mouse monoclonal IgG1, cited in 2 publications, provided at 100 µg/ml
    • recommended for detection of TNFα of human origin by WB, IP and ELISA; non cross-reactive with human TNF β or a panel of other human cytokines
    • available conjugated to FITC for IF, IHC(P) and FCM
    • See TNFα (C-4): sc-133192 for TNFα antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for TNFα Antibody (AS1). This work is in progress.
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For Western Blot, is it recommended to use denatured or non-denatured conditions with TNFα (AS1): sc-52791 antibody?

Asked by: jenniferc
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2020-11-06
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Rated 4 out of 5 by from Works well for WB with good signal Works well for WB with good signal. -SCBT QC
Date published: 2013-05-21
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