Anti-TNF alpha Antibody (4E1) recommended for detection of TNFα of human origin by WB, IF and IHC(P)

Anti-TNFα Antibody (4E1): sc-130349

Anti-TNFα Antibody (4E1) is rated 4.0 out of 5 by 3.
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Datasheets

    • TNFα Antibody (4E1) is a mouse monoclonal IgG1, cited in 25 publications, provided at 200 µg/ml
    • raised against recombinant TNFα of human origin
    • TNF alpha Antibody (4E1) is recommended for detection of TNFα of human origin by WB, IF and IHC(P)
    • Anti-TNF alpha Antibody (4E1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of TNFα (4E1): sc-130349.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for TNFα Antibody (4E1) for WB and IHC(P) applications. These reagents are now offered in bundles with TNFα Antibody (4E1) (see ordering information below).
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Quali cellule usare come controllo positivo e negativo per questo tipo di anticorpo.

Asked by: Andrea1
Thank you for your question. For TNFα (4E1) we suggest HeLa whole cell lysate: sc-2200. f you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: SCBT Heidelberg Support
Date published: 2018-12-21

Can I recieve antibody and IHC kit order in short time ..may be one month..at Iraq.TQ

Asked by: Hazar 123
The lead time and the shipping costs depend on the destination. For more information please contact costumer care by phone or by live chat.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-21

il solvente in cui è disciolto è tipo soluzione fisiologica o solvente organico tipo DMSO? Grazie

Asked by: Andreaa
Molte grazie la sua domanda. Il prodotto sc-130349 contiene 200 g IgG1 in 1.0 ml di PBS con < 0.1% azoturo di sodio azide e 0.1% gelatina. Thank you very much for question. Each vial sc-130349 contains 200 g IgG1 in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin
Answered by: SCBT Heidelberg Support
Date published: 2018-11-27

Can sc-130349: TNFα (4E1) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: cjMara
Thank you for your question. Yes, sc-130349: TNFα (4E1) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 3 out of 5 by from Not so clear on WB The signal was too weak and faded with background .
Date published: 2019-02-21
Rated 5 out of 5 by from great signal various pretreatment regimens tested EDTA gave best signal to background. IHC protocol fully automated on Leica Bond III
Date published: 2017-02-05
Rated 4 out of 5 by from Precise band acquired in immunoblot analysis Precise band acquired in immunoblot analysis. -SCBT QC
Date published: 2013-07-11
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