Anti-TLR4 Antibody (MTS510) recommended for detection of TLR4/MD-2 complex of mouse, rat and human origin by IP, IF and FCM

Anti-TLR4 Antibody (MTS510): sc-13591

Anti-TLR4 Antibody (MTS510) is rated 4.6 out of 5 by 7.
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Datasheets

    • TLR4 Antibody (MTS510) is a rat monoclonal IgG2a, cited in 48 publications, provided at 200 µg/ml
    • raised against Toll-like receptor 4 (TLR4)/MD-2 complex of mouse origin
    • Anti-TLR4 Antibody (MTS510) is recommended for detection of TLR4/MD-2 complex of mouse, rat and human origin by IP, IF and FCM
    • Anti-TLR4 Antibody (MTS510) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to either PerCP or PerCP-Cy5.5 for IF, IHC(P) and FCM
    • also provided azide-free for inhibition of LPS-induced cytokine production, sc-13591 L, 200 µg/0.1 ml
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of TLR4 (MTS510): sc-13591.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for TLR4 Antibody (MTS510). This work is in progress.
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How much is the molecular weight of TLR4 Antibody (MTS510)(sc-13591)? Is the unit of this regent 1ml?

Asked by: Yuanyuan JI
Thank you for your question. The expected molecular weight for TLR4 is 95kDa (120kDa if glycosylated). The product is provided as 200µg in 1ml. Please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Tech Service
Date published: 2018-01-08

What is the epitope recognized by sc-13591: TLR4 (MTS510)?

Asked by: cjMara
LR4 Antibody (MTS510) is a rat monoclonal raised against Toll-like receptor 4 (TLR4)/MD-2 complex of mouse origin. The exact epitope has not been determined.
Answered by: Technical Support 8
Date published: 2017-01-11

What negative control should be used when using the conjugated version of TLR4 (MTS510) monoclonal antibody in flow cytometry?

Asked by: cjMara
Thank you for your inquiry. We recommend using the isotype specific normal rat IgG conjugated to the same fluorophore. For example, if you are using sc-13591 PE, then you would want to use normal rat IgG2a PE, sc-2872.
Answered by: Technical Support 8
Date published: 2017-01-11
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Rated 3 out of 5 by from This antibody works with western blotting This antibody works with western blotting with SAS cells lysate.
Date published: 2020-06-12
Rated 5 out of 5 by from Good antibody Works nicely on mouse and human brain sections (PFA-fixed)
Date published: 2018-06-13
Rated 5 out of 5 by from Good for flow Good TLR4 antibody for flow cytometry. Direct conjugates available to bypass secondary antibody. "L" form is available as azide-free for inhibition of LPS-induced cytokine production.
Date published: 2017-01-23
Rated 5 out of 5 by from Great FCM and IHC Nice product but two alternative TLR4 Monoclonals also from scbt have outstanding data for broader base of applications
Date published: 2017-01-15
Rated 5 out of 5 by from Good results in CoIP with mouse cells Good results in CoIP with mouse cells -SCBT Publication Review
Date published: 2015-04-15
Rated 5 out of 5 by from Excellent for IHC with mouse kidney Excellent for IHC with mouse kidney -SCBT Publication Review
Date published: 2015-02-03
Rated 4 out of 5 by from PE conjugate works very well in Flow PE conjugate works very well in Flow -SCBT QC
Date published: 2013-08-03
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