Anti-TLR4 Antibody (25) recommended for detection of TLR4 of mouse, rat, human and canine origin by WB, IP, IF and IHC(P)

Anti-TLR4 Antibody (25): sc-293072

Anti-TLR4 Antibody (25) is rated 4.7 out of 5 by 95.
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Datasheets

    • Anti-TLR4 Antibody (25) is a mouse monoclonal IgG1 κ TLR4 antibody, cited in 362 publications, provided at 200 µg/ml
    • raised against amino acids 198-395 of TLR4 of mouse origin
    • recommended for detection of TLR4 of mouse, rat, human and canine origin by WB, IP, IF and IHC(P)
    • available conjugated to agarose for IP; and to HRP for WB, IHC(P) and ELISA
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of TLR4 (25): sc-293072.
    • m-IgG Fc BP-HRP is the preferred secondary detection reagent for TLR4 Antibody (25) for WB and IHC(P) applications. This reagent is now offered in a bundle with TLR4 Antibody (25) (see ordering information below).
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do you have a fluorescence tag version of TLR4 Antibody (25): sc-293072 available? or can you order a custom made conjugated version?

Asked by: David Chau
Thank you for your question. TLR4 Antibody (HTA125): sc-13593 and TLR4 Antibody (MTS510): sc-13591 are available in fluorescence-conjugated versions. We do not offer fluorescence conjugates of TLR4 Antibody (25): sc-293072.
Answered by: Tech Support Europe
Date published: 2021-02-09

Is there any chances of double bands in the immunoblot of mouse peritoneal macrophages cells lysates?

Asked by: Soumojit
Thank you for your question. We have not tested this antibody in that specific sample type. We recommend you refer to the product citations using this antibody as a reference. You can search for specific product citations by application, species and sample type using our Product Citation search on our website, or using the link below. https://www.scbt.com/scbt/misc/citationSearch.jsp
Answered by: Technical Support
Date published: 2019-08-17

What is the second antibody??

Asked by: rlaalsgml36
Thank you for your question. You can find our available secondary antibodies here: https://www.scbt.com/scbt/browse/Secondary-Antibodies/_/N-1hoaxk4
Answered by: Tech Service
Date published: 2019-06-24

I didn't get positive result on mouse MC3T3E1 cells by using this TLR4 antibody with1:100 or 1:200 dilution,I was using 555 second antibody,what's the problem is?

Asked by: tommy666
Thank you for your question. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2019-03-04

请问TLR4(25)做免疫组化时抗原修复方法?

Asked by: rainqq
感谢您的提问。我们推荐热修复,详细流程请参考链接: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining Thank you for your question. We recommend heat treatment for antigen retrieval, please visit this link for the detail: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 8
Date published: 2018-12-21

Does this antibody bind in the extracellular or intracellular domain of TLR4?

Asked by: AbPolly
Our TLR4 (25) sc-293072 is raised against amino acids 198-395 of TLR4 of mouse origin (accession # Q9QUK6). This is within the extracellular domain.
Answered by: Technical Service
Date published: 2017-08-01

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: Anna99
We recommend using one of our exclusive new Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding protein conjugates is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 5
Date published: 2016-12-16

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: Anna99
Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This mouse monoclonal TLR4 antibody is currently available conjugated to HRP (sc-293072 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; and to either phycoerythrin (sc-293072 PE) or fluoroscein (sc-293072 FITC), Alexa Fluor® 488 (sc-293072 AF488) or Alexa Fluor® 647 (sc-293072 AF647), 200 µg/ml, for IF, IHC(P) and FCM.
Answered by: Tech Service 5
Date published: 2016-12-15
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Rated 5 out of 5 by from Western blot analysis This TLR4 antibody can be used in ovine lymph node for related research.
Date published: 2021-04-16
Rated 5 out of 5 by from works fine in western blot for THP-1 cells used 20 µg whole cell protein cell lysate of THP-1 cells & antibody 1:500 diluted, incubated at 4°C over night --> get nice bands at ~95kDa (green) (red bands = ß-actin control)
Date published: 2021-02-02
Rated 4 out of 5 by from Sono soddisfatto dell'anticorpo!!!! Abbiamo preso il Trial Kit di questo anticorpo assieme ad un full vial per un altra proteina, per riscontrare se il vostro fosse più efficace nel legarsi alla proteina TLR4 rispetto a quello di altre ditte. Possiamo dire dopo diverse prove che siamo soddisfatti, nonostante non dia un segnale altissimo e richieda degli ECL che siano in grado di far sviluppare proteine che hanno una concentrazione molto bassa. Unica criticità che oltre alla banda che ci si aspetta a 95 KDa si registra un'altra banda intorno ai 50-55 KDa all'altezza della BSA e di norma quella da molto più segnale.
Date published: 2020-12-18
Rated 4 out of 5 by from Funciona muy bien. Probado en células estrelladas de páncreas. Dilucion 1/500. Detecta la proteina.
Date published: 2020-09-22
Rated 5 out of 5 by from TLR4 This is a very good product for WB and for CO-IP test.
Date published: 2020-09-11
Rated 5 out of 5 by from 功能很出色 周围同事购买过该产品,试用后感觉很满意,产品物廉价美,相比其他的产品,在价格以及品质上很有优势,具有购买价值!
Date published: 2020-08-31
Rated 3 out of 5 by from This antibody does not work with western blotting This antibody does not recognize TLR4 with western blotting with SAS cell lysate under reducing condition.
Date published: 2020-06-12
Rated 5 out of 5 by from ottimo Funziona benissimo. Testato in saggio di western blot su proteine totali di linea cellulare di mesotelio pleurico di ratto (4.4 R M4) a diverse diluizioni. Banda pulita intorno a 100 kD
Date published: 2019-04-19
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