TID-1L/S Antibody (RS-13)recommended for detection of TID-1L and TID-1S of mouse, rat and human origin by WB, IP and IF

TID-1L/S Antibody (RS-13): sc-18819

TID-1L/S Antibody (RS-13) is rated 5.0 out of 5 by 4.
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Datasheets

    • TID-1L/S Antibody (RS-13) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against human recombinant TID-1
    • TID-1L/S Antibody (RS-13) is recommended for detection of TID-1L and TID-1S of mouse, rat and human origin by WB, IP and IF
    • TID-1L/S Antibody (RS-13) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of TID-1L/S (RS-13): sc-18819.
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What is the recommended fixation method for immunofluorescence staining with TID-1L/S (RS-13): sc-18819 monoclonal antibody?

Asked by: jerojero
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Super anticorps Cet anticorps fonctionne très bien en western blot et en IP. Testé en western blot sur 13 µg d'extraits totaux de cellules COV434. Dilution au 1/200 en TBT-lait.
Date published: 2017-10-11
Rated 5 out of 5 by from Publishable IP and WB data with human and mouse PC Publishable IP and WB data with human and mouse PC-12, 293T and neurons - SCBT Publication Review
Date published: 2015-02-08
Rated 5 out of 5 by from Produced excellent immunofluorescence cytoplasmic Produced excellent immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-01-26
Rated 5 out of 5 by from WB publishable data with U2OS and mouse brain WB publishable data with U2OS and mouse brain tissue - SCBT Publication Review
Date published: 2013-01-10
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