Anti-TID-1L/S Antibody (RS-13) is a mouse monoclonal IgG1 (kappa light chain) TID-1L/S antibody provided at 200 µg/ml
raised against human recombinant TID-1
Anti-TID-1L/S Antibody (RS-13) is recommended for detection of TID-1L and TID-1S of mouse, rat and human origin by WB, IP and IF
Anti-TID-1L/S Antibody (RS-13) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of TID-1L/S (RS-13): sc-18819.
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Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
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Labware ships FedEx Ground free of charge to the contiguous US
TID-1L/S Antibody (RS-13) is a high quality monoclonal TID-1L/S antibody (also designated TID-1L/S antibody) suitable for the detection of the TID-1L/S protein of mouse, rat and human origin. TID-1L/S Antibody (RS-13) is available as both the non-conjugated anti-TID-1L/S antibody form, as well as multiple conjugated forms of anti-TID-1L/S antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. TID-1 is the human homologue of the Drosophila tumor suppressor protein TID56. Both TID56 and TID-1 belong to the DnaJ family of proteins, which are characterized by a highly conserved J domain that influences apoptotic activity. The human TID-1 gene encodes two splice variants, TID-1L and TID-1S TID-1L expression increases apoptosis, whereas a mutant J domain suppresses apoptosis. By contrast, TID-1S expression suppresses apoptosis, whereas a mutant J domain increases apoptosis. TID-1L and TID-1S are localized to the mitochondrial matrix, where they regulate apoptotic signal transduction by affecting cytochrome c release and caspase-3 activation. Both TID-1L and TID-1S are cleaved at amino acid 66 upon entry into the mitochondria, indicating that mature TID-1L and TID-1S represent cleavage products of cytoplasmic pre-proteins.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
What is the recommended fixation method for immunofluorescence staining with TID-1L/S (RS-13): sc-18819 monoclonal antibody?
Asked by: jerojero
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here:
Rated 5 out of
NL LGBC from
Super anticorpsCet anticorps fonctionne très bien en western blot et en IP. Testé en western blot sur 13 µg d'extraits totaux de cellules COV434. Dilution au 1/200 en TBT-lait.
Date published: 2017-10-11
Rated 5 out of
Publishable IP and WB data with human and mouse PCPublishable IP and WB data with human and mouse PC-12, 293T and neurons - SCBT Publication Review
Date published: 2015-02-08
Rated 5 out of
Produced excellent immunofluorescence cytoplasmicProduced excellent immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-01-26
Rated 5 out of
WB publishable data with U2OS and mouse brainWB publishable data with U2OS and mouse brain tissue - SCBT Publication Review
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