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Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
TID-1 is the human homologue of the Drosophila tumor suppressor protein TID56. Both TID56 and TID-1 belong to the DnaJ family of proteins, which are characterized by a highly conserved J domain that influences apoptotic activity. The human TID-1 gene encodes two splice variants, TID-1L and TID-1S TID-1L expression increases apoptosis, whereas a mutant J domain suppresses apoptosis. By contrast, TID-1S expression suppresses apoptosis, whereas a mutant J domain increases apoptosis. TID-1L and TID-1S are localized to the mitochondrial matrix, where they regulate apoptotic signal transduction by affecting cytochrome c release and caspase-3 activation. Both TID-1L and TID-1S are cleaved at amino acid 66 upon entry into the mitochondria, indicating that mature TID-1L and TID-1S represent cleavage products of cytoplasmic pre-proteins.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
What is the recommended fixation method for immunofluorescence staining with TID-1L/S (RS-11): sc-18820 monoclonal antibody?
Asked by: jerojero
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here:
Rated 5 out of
Produced superb immunofluorescence cytoplasmicProduced superb immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-03-19
Rated 5 out of
IP and WB and IF publishable data with human MCFIP and WB and IF publishable data with human MCF-7, T47D, SK-BR-3, BT474, human glioma cell lines, , U87, U373, HCT 116cells - SCBT Publication Review
To place an order using RMB or to ship to mainland China, please visit www.scbio.cn
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