Anti-TGF beta RIII Antibody (A-4) recommended for detection of TGFβ RIII of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-TGFβ RIII Antibody (A-4): sc-74511

Anti-TGFβ RIII Antibody (A-4) is rated 4.6 out of 5 by 7.
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Datasheets

    • Anti-TGFβ RIII Antibody (A-4) is a mouse monoclonal IgG1 κ TGFβ RIII antibody, cited in 6 publications, provided at 200 µg/ml
    • raised against amino acids 511-790 mapping near the C-terminus of TGFβ RIII of human origin
    • Anti-TGF beta RIII Antibody (A-4) is recommended for detection of TGFβ RIII of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-TGF beta RIII Antibody (A-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of TGFβ RIII (A-4): sc-74511.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for TGFβ RIII Antibody (A-4) for WB and IHC(P) applications. These reagents are now offered in bundles with TGFβ RIII Antibody (A-4) (see ordering information below).
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Can TGFβ RIII (A-4): sc-74511 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Dr Ninau Qelp
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, TGFβ RIII (A-4): sc-74511 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from Produces multiple bands in airway smooth muscle Western using 16ug of total protein from human airway smooth muscle cells produced multiple bands (bands at 60, 150 and 250 kDa were the strongest with additional bands at 40 & 100 kDa).
Date published: 2017-07-31
Rated 5 out of 5 by from 性价比高 文献中推荐了该抗体,自己用,果然不错,推荐他人购买!比其他公司的抗体,价格实惠,量也够用!相信该品牌!
Date published: 2017-06-07
Rated 5 out of 5 by from Excellent! This antibody is great in Western blot analysis of human recombinant TGFβ RIII under reducing conditions.
Date published: 2017-01-26
Rated 5 out of 5 by from Good WB analysis It works well to detect TGFBR3 in total protein of nucleus pulposus cells, with the dilution ration 1:500.
Date published: 2016-11-23
Rated 5 out of 5 by from Nice membrane and cytoplasmic staining of Nice membrane and cytoplasmic staining of glomerular cells and cytoplasmic staining of cells in tubules of formalin fixed, paraffin-embedded human kidney tissue. - SCBT QC
Date published: 2015-03-16
Rated 5 out of 5 by from Good Western blot analysis of TGFb RIII expression Good Western blot analysis of TGFb RIII expression in ECV304 whole cell lysate. - SCBT QC
Date published: 2015-01-04
Rated 4 out of 5 by from Satisfactory Western blot analysis of human Satisfactory Western blot analysis of human recombinant TGFb RIII under reducing conditions. - SCBT QC
Date published: 2013-06-11
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