Anti-TFIIF RAP 30 Antibody (G-3) recommended for detection of TFIIF RAP 30 of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-TFIIF RAP 30 Antibody (G-3): sc-374304

Anti-TFIIF RAP 30 Antibody (G-3) is rated 4.7 out of 5 by 3.
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Datasheets

    • Anti-TFIIF RAP 30 Antibody (G-3) is a mouse monoclonal IgG1 (kappa light chain) TFIIF RAP 30 antibody provided at 200 µg/ml
    • raised against amino acids 102-249 mapping near the C-terminus of TFIIF RAP 30 of human origin
    • Anti-TFIIF RAP 30 Antibody (G-3) is recommended for detection of TFIIF RAP 30 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-TFIIF RAP 30 Antibody (G-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for ChIP application (sc-374304 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of TFIIF RAP 30 (G-3): sc-374304.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for TFIIF RAP 30 Antibody (G-3) for WB applications. These reagents are now offered in bundles with TFIIF RAP 30 Antibody (G-3) (see ordering information below).
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What is the recommended fixation method for immunofluorescence staining with TFIIF RAP 30 (G-3): sc-374304 monoclonal antibody?

Asked by: jenniferc
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Strong robust bands observedy by Western Strong robust bands observedy by Western Blot at the expected molecular weight for TFIIF RAP 30 protein using K-562, Jurkat, A-431, HeLa and U-937 nuclear extracts. -SCBT QC
Date published: 2015-05-30
Rated 5 out of 5 by from Produced beautiful immunofluorescence nuclear Produced beautiful immunofluorescence nuclear staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-04-30
Rated 4 out of 5 by from Positive detection of endogenously expressed Positive detection of endogenously expressed TFIIF RAP 30 protein in HeLa cells through analysis by Western Blot. -SCBT QC
Date published: 2015-02-10
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