Anti-TFIIE-beta Antibody (A-1) recommended for detection of TFIIE-β p34 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-TFIIE-β Antibody (A-1): sc-137000

Anti-TFIIE-β Antibody (A-1) is rated 5.0 out of 5 by 3.
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Datasheets

    • Anti-TFIIE-β Antibody (A-1) is a mouse monoclonal IgG2b κ TFIIE-β antibody, cited in 2 publications, provided at 200 µg/ml
    • raised against amino acids 1-291 of TFIIE-β of human origin
    • Anti-TFIIE-beta Antibody (A-1) is recommended for detection of TFIIE-β p34 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-TFIIE-beta Antibody (A-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for ChIP application (sc-137000 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of TFIIE-β (A-1): sc-137000.
    • m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for TFIIE-β Antibody (A-1) for WB and IHC(P) applications. These reagents are now offered in bundles with TFIIE-β Antibody (A-1) (see ordering information below).
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When should the TransCruz reagent sc-137000 X be used instead of the standard form sc-137000?

Asked by: Trav11
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-28
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Rated 5 out of 5 by from Good specificity shown by using transfected Good specificity shown by using transfected and non-transfected 293T cells for over-expression of the mouse TFIIE- protein via Western Blot analysis. -SCBT QC
Date published: 2015-05-07
Rated 5 out of 5 by from Positive detection of endogenously expressed TFIIE Positive detection of endogenously expressed TFIIE- protein in HeLa and HL-60 whole cell lysates through analysis by Western Blot. -SCBT QC
Date published: 2015-01-31
Rated 5 out of 5 by from Great immunoperoxidase nuclear staining in Great immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human pancreas tissue. -SCBT QC
Date published: 2015-01-28
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