Anti-TFIID/TATA Binding Protein/TBP Antibody (ITBP20) recommended for detection of TFIID of mouse, rat and human origin by WB and IP

Anti-TFIID/TATA Binding Protein/TBP Antibody (ITBP20): sc-56796

Anti-TFIID/TATA Binding Protein/TBP Antibody (ITBP20) is rated 4.3 out of 5 by 4.
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Datasheets

    • TFIID/TATA Binding Protein/TBP Antibody (ITBP20) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
    • raised against recombinant TFIID of human origin
    • recommended for detection of TFIID of mouse, rat and human origin by WB and IP
    • See TFIID/TATA Binding Protein/TBP (58C9): sc-421 for TFIID/TATA Binding Protein/TBP antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for TFIID/TATA Binding Protein/TBP Antibody (ITBP20) for WB applications. These reagents are now offered in bundles with TFIID/TATA Binding Protein/TBP Antibody (ITBP20) (see ordering information below).
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What blocking solution should I use with TFIID (ITBP20): sc-56796 monoclonal antibody for Western blot?

Asked by: jerojero
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 4 out of 5 by from Positive detection of endogenously expressed Positive detection of endogenously expressed TFIID protein in F9 and 293T whole cell lysate and rat testis tissue extract through analysis by Western Blot. -SCBT QC
Date published: 2015-03-17
Rated 5 out of 5 by from Good specificity shown by using transfected Good specificity shown by using transfected and non-transfected 293T cells for over-expression of the human TFIID protein via Western Blot analysis. -SCBT QC
Date published: 2015-01-31
Rated 4 out of 5 by from Expression levels of the TFIID protein in Expression levels of the TFIID protein in various cell lines demonstrated by WB: SK-N-MC, Hep G2 and K-562 nuclear extracts.
Date published: 2015-01-03
Rated 4 out of 5 by from Published WB data with human HEp Published WB data with human HEp-2 cells - SCBT Publication Review
Date published: 2014-03-07
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