Anti-TFIID/TATA Binding Protein/TBP Antibody (58C9) recommended for detection of TATA binding protein (TBP) of mouse, rat and human origin by WB, IP and IF

Anti-TFIID/TATA Binding Protein/TBP Antibody (58C9): sc-421

Anti-TFIID/TATA Binding Protein/TBP Antibody (58C9) is rated 4.9 out of 5 by 14.
  • y_2021, m_10, d_19, h_18
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_14
  • loc_en_US, sid_421, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 131ms
  • REVIEWS, PRODUCT
loading
5
12
4
2
3
0
2
0
1
0

Datasheets

    • Anti-TFIID/TATA Binding Protein/TBP Antibody (58C9) is a mouse monoclonal IgG2b TFIID/TATA Binding Protein/TBP antibody, cited in 70 publications, provided at 200 µg/ml
    • raised against bacterially produced dTBP
    • Anti-TFIID/TATA Binding Protein/TBP Antibody (58C9) is recommended for detection of TATA binding protein (TBP) of mouse, rat and human origin by WB, IP and IF
    • Anti-TFIID/TATA Binding Protein/TBP Antibody (58C9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-421 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of TFIID/TATA Binding Protein/TBP (58C9): sc-421.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for TFIID/TATA Binding Protein/TBP Antibody (58C9). This work is in progress.
loading

I would like to use this antibody for EMSA (Gelshift), would you recommend using the regular form or the x-form of the antibody (TransCruz reagent)?

Asked by: TinTin
We recommend using the TransCruz reagent form, sc-421 X, when performing ChIP or EMSA assays. This more concetrated form lets you use a smaller volume, which is desired when doing EMSA experiments. The x-form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support 7
Date published: 2017-01-22
  • y_2021, m_10, d_17, h_18CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_en_US, sid_421, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 108ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from Recommended for western blotting! It detected endogenous TBP in lysates from HEK 293T cells (1:500 dilution in 5% milk in TBS-0.1% Tween-20, overnight at 4C).
Date published: 2018-06-18
Rated 4 out of 5 by from Good for WB but weak for ChIP This antibody is good for Western blot but only produces weak enrichment for ChIP.
Date published: 2018-05-19
Rated 5 out of 5 by from Works for ChIP! The 58C9 clone is published for ChIP. See PMID: 12612078 & 22441827 as examples.
Date published: 2016-12-22
Rated 5 out of 5 by from It has been reported that this antibody is It has been reported that this antibody is suitable for use in EMSA applications using human samples. -SCBT Publication Review
Date published: 2015-06-22
Rated 5 out of 5 by from Publishable data obtained by ChIP using human Publishable data obtained by ChIP using human HeLa cells. -SCBT Publication Review
Date published: 2015-05-25
Rated 5 out of 5 by from Positive detection of endogenously expressed Positive detection of endogenously expressed TFIID protein in NIH/3T3, HeLa and F9 cells through analysis by Western Blot. -SCBT QC
Date published: 2015-05-24
Rated 5 out of 5 by from An increase in signal was observed via Western An increase in signal was observed via Western Blot when comparing mouse TFIID transfected 293T cells with those that have not been transfected. -SCBT QC
Date published: 2015-05-22
Rated 5 out of 5 by from Good specificity shown by using transfected Good specificity shown by using transfected and non-transfected 293T cells for over-expression of the human TFIID protein via Western Blot analysis. -SCBT QC
Date published: 2015-05-18
  • y_2021, m_10, d_19, h_18
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_14
  • loc_en_US, sid_421, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 7ms
  • REVIEWS, PRODUCT