Anti-TFIID/TATA Binding Protein/TBP Antibody (1TB18) recommended for detection of TFIID of mouse, rat and human origin by WB, IP, IF and IHC(P)

Anti-TFIID/TATA Binding Protein/TBP Antibody (1TB18): sc-56794

Anti-TFIID/TATA Binding Protein/TBP Antibody (1TB18) is rated 5.0 out of 5 by 4.
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Datasheets

    • TFIID/TATA Binding Protein/TBP Antibody (1TB18) is a mouse monoclonal IgG1 κ, cited in 4 publications, provided at 200 µg/ml
    • raised against a synthetic TFIID peptide of human origin
    • recommended for detection of TFIID of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • See TFIID/TATA Binding Protein/TBP (58C9): sc-421 for TFIID/TATA Binding Protein/TBP antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for TFIID/TATA Binding Protein/TBP Antibody (1TB18) for WB and IHC(P) applications. These reagents are now offered in bundles with TFIID/TATA Binding Protein/TBP Antibody (1TB18) (see ordering information below).
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I am using TFIID (1TB18): sc-56794 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Germaine
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Specificity of this TFIID antibody was demonstrated Specificity of this TFIID antibody was demonstrated by WB by comparing human TFIID transfected 293T cells with those that have not been transfected. -SCBT QC
Date published: 2015-06-30
Rated 5 out of 5 by from Good specificity and signal strength in F9 Good specificity and signal strength in F9 whole cell lysate and rat testis tissue extract. -SCBT QC
Date published: 2015-05-30
Rated 5 out of 5 by from Superb immunoperoxidase nuclear staining Superb immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human cerebral cortex tissue. -SCBT QC
Date published: 2015-04-18
Rated 5 out of 5 by from Clean blot with single band at expected size Clean blot with single band at expected size using SK-N-MC, Hep G2 and K-562 nuclear extracts. -SCBT QC
Date published: 2015-01-25
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