Anti-TFIIB Antibody (IIB8) recommended for detection of TFIIB of mouse, rat and human origin by WB and IP

Anti-TFIIB Antibody (IIB8): sc-23875

Anti-TFIIB Antibody (IIB8) is rated 4.3 out of 5 by 3.
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Datasheets

    • TFIIB Antibody (IIB8) is a mouse monoclonal IgG2a κ, cited in 11 publications, provided at 200 µg/ml
    • raised against partially purified recombinant human TFIIB, epitope maps to amino acids 52-105
    • recommended for detection of TFIIB of mouse, rat and human origin by WB and IP
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-23875 X, 200 µg/0.1 ml)
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for TFIIB Antibody (IIB8) for WB applications. These reagents are now offered in bundles with TFIIB Antibody (IIB8) (see ordering information below).
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When should the TransCruz reagent sc-23875 X be used instead of the standard form sc-23875?

Asked by: jenniferc
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from It has been reported in literature that this It has been reported in literature that this antibody is suitable for use in WB using human Jurkat and SNU601 cells. -SCBT Publication Review
Date published: 2015-05-28
Rated 4 out of 5 by from Positive detection of endogenously expressed Positive detection of endogenously expressed TFIIB protein in K-562 nuclear extract and Hep G2 whole cell lysate through analysis by Western Blot. -SCBT QC
Date published: 2015-05-24
Rated 4 out of 5 by from Good specificity shown by using transfected Good specificity shown by using transfected and non-transfected 293T cells for over-expression of the mouse TFIIB protein via Western Blot analysis. -SCBT QC
Date published: 2015-02-15
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