Anti-TFIIB Antibody (D-3) recommended for detection of TFIIB p33 of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-TFIIB Antibody (D-3): sc-271736

Anti-TFIIB Antibody (D-3) is rated 4.0 out of 5 by 7.
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Datasheets

    • Anti-TFIIB Antibody (D-3) is a mouse monoclonal IgG1 κ TFIIB antibody, cited in 14 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 289-318 near the C-terminus of TFIIB of human origin
    • Anti-TFIIB Antibody (D-3) is recommended for detection of TFIIB p33 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and canine, bovine and porcine
    • TransCruz reagent for ChIP application (sc-271736 X, 200 µg/0.1 ml)
    • Anti-TFIIB Antibody (D-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of TFIIB (D-3): sc-271736.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for TFIIB Antibody (D-3) for WB applications. These reagents are now offered in bundles with TFIIB Antibody (D-3) (see ordering information below).
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What application is the blocking peptide sc-271736 P appropriate for?

Asked by: AbPolly
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-03-01
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Rated 1 out of 5 by from Not for ChIP We only tried it for ChIP. It is clearly unsuitable for ChIP.
Date published: 2020-07-10
Rated 5 out of 5 by from 免疫沈降で使用。 Immunoprecipitationで使用。5ul/sampleで用いた。良好に免疫沈降することができた。
Date published: 2020-06-19
Rated 3 out of 5 by from Good I used this antibody for c2c12 cell and small intestine sample. In c2c12, it was good but not good for intestine sample.
Date published: 2020-03-19
Rated 5 out of 5 by from good antibody TFIIB(D-3) mouse monoclonal antibody 1:200 diluted, the results show that the antibody works well.
Date published: 2018-08-21
Rated 4 out of 5 by from good it works well, but the rusult doesn't show up within my predict. maybe my sample does not work well. I should change my condition.
Date published: 2017-09-25
Rated 5 out of 5 by from Superb immunofluorescence nucleolar staining Superb immunofluorescence nucleolar staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-01-05
Rated 5 out of 5 by from Good Western Blot data of TFIIB expression in K Good Western Blot data of TFIIB expression in K-562 and Jurkat whole cell lysates. -SCBT QC
Date published: 2014-11-03
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