Anti-TFIIB Antibody (24) recommended for detection of TFIIB of mouse, rat, human and canine origin by WB, IP and IF

Anti-TFIIB Antibody (24): sc-136190

Anti-TFIIB Antibody (24) is rated 5.0 out of 5 by 3.
  • y_2021, m_10, d_19, h_18
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_3
  • loc_en_US, sid_136190, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 123ms
  • REVIEWS, PRODUCT
loading
5
3
4
0
3
0
2
0
1
0

Datasheets

    • TFIIB Antibody (24) is a mouse monoclonal IgG2a, cited in 1 publications, provided at 50 µg/0.5 ml
    • raised against amino acids 2-155 of TFIIB of human origin
    • recommended for detection of TFIIB of mouse, rat, human and canine origin by WB, IP and IF
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for TFIIB Antibody (24). This work is in progress.
loading

I am using TFIIB (24): sc-136190 for immunofluorescence, what dilution of this antibody should I use?

Asked by: jerojero
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
  • y_2021, m_10, d_19, h_18CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_en_US, sid_136190, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 163ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Positive detection of endogenously expressed Positive detection of endogenously expressed TFIIB protein in Jurkat, HeLa, K-562, Hep G2 and U-937 nuclear extracts through analysis by Western Blot. -SCBT QC
Date published: 2015-05-28
Rated 5 out of 5 by from Published WB data with human fractionated Published WB data with human fractionated cell lysate - SCBT Publication Review
Date published: 2015-03-28
Rated 5 out of 5 by from Excellent immunofluorescence nuclear staining Excellent immunofluorescence nuclear staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2014-04-30
  • y_2021, m_10, d_19, h_18
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_3
  • loc_en_US, sid_136190, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 16ms
  • REVIEWS, PRODUCT