Anti-TFII-I Antibody (B-7) recommended for detection of short and long isoforms of TFII-I of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-TFII-I Antibody (B-7): sc-46670

Anti-TFII-I Antibody (B-7) is rated 4.5 out of 5 by 4.
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Datasheets

    • Anti-TFII-I Antibody (B-7) is a mouse monoclonal IgG1 κ TFII-I antibody, cited in 2 publications, provided at 200 µg/ml
    • raised against amino acids 941-998 of TFII-I of human origin
    • Anti-TFII-I Antibody (B-7) is recommended for detection of short and long isoforms of TFII-I of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-TFII-I Antibody (B-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-46670 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of TFII-I (B-7): sc-46670.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for TFII-I Antibody (B-7) for WB and IHC(P) applications. These reagents are now offered in bundles with TFII-I Antibody (B-7) (see ordering information below).
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When should the TransCruz reagent sc-46670 X be used instead of the standard form sc-46670?

Asked by: jerojero
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from WB能识别目的条带 WB1:1000稀释,我是4℃过夜孵育的,目的条带亮度可以。内源条带单一,位置在130KD左右,有不同剪切体。但是只识别人源,鼠源信号几乎没有。
Date published: 2018-02-26
Rated 5 out of 5 by from Worked great for Western and immunoprecipitation. This TFII-I monoclonal detected TFII-I in transiently transfected 293T cell lysates (1:1000 dilution). It also immunoprecipitated TFII-I from the same lysates (2 microgram antibody / 100 micoliter lysate).
Date published: 2017-06-05
Rated 4 out of 5 by from Good specificity and signal strength in HeLa Good specificity and signal strength in HeLa nuclear extract. -SCBT QC
Date published: 2015-06-22
Rated 5 out of 5 by from Produced exceptional immunoperoxidase nuclear Produced exceptional immunoperoxidase nuclear and cytoplasmic staining in formalin fixed, paraffin-embedded human esophagus tissue. -SCBT QC
Date published: 2015-03-11
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