TFEB Antibody (C-6) recommended for detection of TFEB of human origin by WB, IP, IF and ELISA

TFEB Antibody (C-6): sc-166736

TFEB Antibody (C-6) is rated 4.6 out of 5 by 11.
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Datasheets

    • Anti-TFEB Antibody (C-6) is a mouse monoclonal IgG1 (kappa light chain) TFEB antibody provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 455-470 within an internal region of TFEB of human origin
    • TFEB Antibody (C-6) is recommended for detection of TFEB of human origin by WB, IP, IF and ELISA
    • Anti-TFEB Antibody (C-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for ChIP application (sc-166736 X, 200 µg/0.1 ml)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of TFEB (C-6): sc-166736.

是否可以用于大鼠的组织或细胞

Asked by: pearsun
Thank you for your inquiry. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website. 感谢您的提问。请将您的目的蛋白序列发送到我们的技术支持部门。我们将对比您的序列以及我们的数据,以解答您物种反应性的相关问题。亚洲技术支持电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”。
Answered by: Technical Support 9
Date published: 2019-02-19

What application is the blocking peptide sc-166736 P appropriate for?

Asked by: DefinitelyNotMatt
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from TFEB C6 sample antibody tested with 1/500 dilution and o/n 4°C. gave a signal of correct size in HeLa whole cell lysate, but did not work for HCT8 cell line.
Date published: 2018-02-16
Rated 5 out of 5 by from 效果不错 我用了TFEB(C-6)试用小样,免疫组化标记的结果比较特异,t(6;11)染色体易位石蜡包埋组织细胞核阳性。
Date published: 2017-10-25
Rated 4 out of 5 by from GOOD PICTURE I get a free sample. Chicken brain tissue , 1:200, NC, after several adjustment on protein sample , get the target line and no other lines.
Date published: 2017-09-26
Rated 5 out of 5 by from specific band Good detection for endogenous detection of TFEB by WB (1:800, 5% BSA,TBST),we got specific band, no background signal,the condition should be optimized.
Date published: 2017-09-02
Rated 4 out of 5 by from Works on overexpressed/endogenous TFEB (WB, 293A) Works on overexpressed/endogenous TFEB (WB, 293A). RIPA extracted protein post 1-6 h serum starvation, weak endogenous band at ~70 kDa. 25 ug spl, LI-COR detection
Date published: 2017-04-04
Rated 5 out of 5 by from Highly recommend This antibody is great! Nice solid bands on Western blot, specific IF staining results, too. Highly recommend .
Date published: 2017-03-10
Rated 5 out of 5 by from Can be used in many cell lines. We got specific signal for the protein at the right molecular weight.
Date published: 2017-02-08
Rated 5 out of 5 by from Worked well in western blot We used this antibody for western blot and got a band when looking at HeLa cells
Date published: 2017-01-16
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