Anti-TF Antibody (H-9) recommended for detection of TF of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-TF Antibody (H-9): sc-374441

Anti-TF Antibody (H-9) is rated 4.2 out of 5 by 6.
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Datasheets

    • Anti-TF Antibody (H-9) is a mouse monoclonal IgG2b κ TF antibody, cited in 12 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 125-163 within an internal region of TF of mouse origin
    • Anti-TF Antibody (H-9) is recommended for detection of TF of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-TF Antibody (H-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of TF (H-9): sc-374441.
    • m-IgGκ BP-HRP is the preferred secondary detection reagent for TF Antibody (H-9) for WB and IHC(P) applications. This reagent is now offered in a bundle with TF Antibody (H-9) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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What application is the blocking peptide sc-374441 P appropriate for?

Asked by: Trav11
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Dr Sadek Ab work good with dilution 1:200 and 2 hrs incubation at 37ºc and overnight at 4 ºc
Date published: 2018-11-21
Rated 4 out of 5 by from Working well in WB This TF antibody works well in mouse brain tissues. Clean background. However, its size is a little off.
Date published: 2017-11-03
Rated 4 out of 5 by from TF The TF Antibody works very well for Western blot applications, showing strong and clear banding with minimal background noise present
Date published: 2017-07-31
Rated 5 out of 5 by from IF worked pretty good in IF with PFA fixed human mesechymal stem cells
Date published: 2016-12-27
Rated 4 out of 5 by from Worked well with transfected lysate by WB Worked well with transfected lysate by WB. -SCBT QC
Date published: 2015-03-30
Rated 4 out of 5 by from Strong bands seen in WB using JEG Strong bands seen in WB using JEG-3, WI-38 and A-431 whole cell lysates and human kidney tissue extract. -SCBT QC
Date published: 2013-06-20
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