Anti-TCR V beta 8.5 Antibody (B73) recommended for detection of TCR V β 8.5 of mouse and rat origin by FCM

Anti-TCR V β 8.5 Antibody (B73): sc-59388

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Anti-TCR V β 8.5 Antibody (B73) is rated 2.0 out of 5 by 1.
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Datasheets

    • TCR V β 8.5 Antibody (B73) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against T cell clone expressing TCR V β8.5 of rat origin
    • recommended for detection of TCR V β 8.5 of mouse and rat origin by FCM
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for TCR V β 8.5 Antibody (B73) for WB and IHC(P) applications. These reagents are now offered in bundles with TCR V β 8.5 Antibody (B73) (see ordering information below).
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How can TCR V β 8.5 (B73): sc-59388 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: Germaine
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to phycoerythrin (sc-59388 PE) or fluoroscein (sc-59388 FITC).
Answered by: Technical Support
Date published: 2017-03-01
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