Anti-TCR C beta 1 Antibody (JOVI.1) recommended for detection of TCR C β 1 of human origin by IP, IF and FCM

Anti-TCR C β 1 Antibody (JOVI.1): sc-53196

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Anti-TCR C β 1 Antibody (JOVI.1) is rated 4.0 out of 5 by 2.
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Datasheets

    • Anti-TCR C β 1 Antibody (JOVI.1) is a mouse monoclonal IgG2a κ TCR C β 1 antibody, cited in 1 publications, provided at 200 µg/ml
    • raised against thymus, spleen and mesenteric lymph nodes isolated from a mouse transgenic for human Vb3 TcR
    • TCR C beta 1 Antibody (JOVI.1) is recommended for detection of TCR C β 1 of human origin by IP, IF and FCM
    • TCR C beta 1 Antibody (JOVI.1) is available conjugated to agarose for IP; to HRP for WB, IHC(P) and ELISA; and to FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of TCR C β 1 (JOVI.1): sc-53196.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for TCR C β 1 Antibody (JOVI.1) for WB and IHC(P) applications. These reagents are now offered in bundles with TCR C β 1 Antibody (JOVI.1) (see ordering information below).
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Can TCR C β 1 (JOVI.1): sc-53196 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, TCR C β 1 (JOVI.1): sc-53196 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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