Anti-TCR beta Antibody (G-11) recommended for detection of TCR β of human origin by WB, IP, IF, IHC(P) and ELISA

Anti-TCR β Antibody (G-11): sc-5277

Anti-TCR β Antibody (G-11) is rated 4.9 out of 5 by 10.
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Datasheets

    • TCR β Antibody (G-11) is a mouse monoclonal IgG1 κ, cited in 10 publications, provided at 200 µg/ml
    • raised against the constant region of TCR β of human origin
    • TCR beta Antibody (G-11) is recommended for detection of TCR β of human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-TCR beta Antibody (G-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of TCR β (G-11): sc-5277.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for TCR β Antibody (G-11) for WB and IHC(P) applications. These reagents are now offered in bundles with TCR β Antibody (G-11) (see ordering information below).
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Does it recognize mouse TCR beta or only human

Asked by: Maria Teresa
Thank you for your question. This antibody has not been tested for mouse and rat use, and can therefore not be warrantied for this use. If you have any further questions, please contact Technical Service.
Answered by: Tech Service
Date published: 2019-03-01

I tried this Ab for IF with dilution 1:50 on 4% PFA fixed Jurkat cells but I didn't see the specific staining. How should I troubleshoot it?

Asked by: KanyaratP
Thank you for your question. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2019-01-14

Any idea how much to use TCR β Antibody (G-11) for Flow Cytometry? I tested at final staining concentration 5ug/ml and failed to see any positive stain in purified human T-cells.

Asked by: JonathanH
Thank you for your question. Below is a link to our recommended protocol for flow cytometry. Please contact our Technical Service Department if you would like help troubleshooting the antibody. Please call us toll-free at (800) 457-3801 option 2, e-mail <[email protected]>, or use the live chat on our website. https://www.scbt.com/scbt/resources/protocols/flow-cytometry
Answered by: Technical Support
Date published: 2017-10-12

Does TCR β (G-11): sc-5277 react with canine samples?

Asked by: Trav11
Thank you for your question. For additional species reactivity not listed on our website, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or by using the Live Chat function on our website and we will be more than happy to run sequence analysis for your species of interest.
Answered by: Technical Support 15
Date published: 2017-01-16

Why is TCR β (G-11): sc-5277 the recommended alternative to the now discontinued TCR β (H-197): sc-9101?

Asked by: Trav11
Thank you for your question. The monoclonal primary antibody TCR β (G-11): sc-5277 offers a higher degree of specificity and essentially eliminates all lot-to-lot variation. It is also warrantied for all of the same applications as the discontinued polyclonal antibody: WB, IP, IF, and IHC(P). If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Service 11
Date published: 2017-01-16
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Rated 5 out of 5 by from 还不错 虽然订购过程很郁闷,但是真正发货还是很快到货的。使用的时候基本上按照说明书的稀释比使用,基本能满足要求
Date published: 2021-04-06
Rated 5 out of 5 by from IHC I do IHC with this antibody on human brain sections. I got excellent specific staining of interneurons without any background. The antibody dilution: 1:200.
Date published: 2017-08-19
Rated 5 out of 5 by from The sc-5277 TCR beta antibody has good specificity, it detect this sample from dilution 1:500, clean background.
Date published: 2017-05-07
Rated 5 out of 5 by from IHC-P This antibody could be used to do IHC-P in human samples.
Date published: 2017-02-10
Rated 5 out of 5 by from Outstanding product !! Better than polyclonal alternative for WB, IF and IHC applications
Date published: 2017-01-20
Rated 5 out of 5 by from Good for IHC and IF This TCR beta antibody shows good staining for IF and IHC in human samples.
Date published: 2017-01-12
Rated 5 out of 5 by from Robust bands in western blot analysis of Robust bands in western blot analysis of TCR expression in MOLT-4 and CCRF-CEM whole cell lysates. -SCBT QC
Date published: 2015-06-30
Rated 5 out of 5 by from Great IF images using this antibody with methanol Great IF images using this antibody with methanol-fixed CCRF-CEM cells -SCBT QC
Date published: 2015-06-16
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