Anti-TCR Antibody (H57-597) recommended for detection of a common epitope of the β chain of the T-cell Receptor (TCR) complex on α/β TCR expressing T lymphocytes and thymocytes of mouse origin by IP, IF and FCM

Anti-TCR Antibody (H57-597): sc-18904

Anti-TCR Antibody (H57-597) is rated 4.3 out of 5 by 3.
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Datasheets

    • TCR Antibody (H57-597) is a Armenian hamster monoclonal IgG, cited in 4 publications, provided at 200 µg/ml
    • raised against affinity purified murine T cell receptor
    • recommended for detection of a common epitope of the β chain of the T-cell Receptor (TCR) complex on α/β TCR expressing T lymphocytes and thymocytes of mouse origin by IP, IF and FCM; non cross-reactive with gd TCR-bearing T cells
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also provided azide-free for stimulation of T cells, sc-18904 L, 200 µg/0.1 ml
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for TCR Antibody (H57-597). This work is in progress.
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How can TCR (H57-597): sc-18904 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: jenniferc
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to phycoerythrin (sc-18904 PE) or fluoroscein (sc-18904 FITC).
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Great antibody for FCM it is great in FCM for primary mouse spleen cells . Low background signal.
Date published: 2016-12-20
Rated 4 out of 5 by from Good FCM data of mouse peripheral blood leukocytes Good FCM data of mouse peripheral blood leukocytes. -SCBT QC
Date published: 2015-06-01
Rated 4 out of 5 by from Great indirect FCM data of TK Great indirect FCM data of TK-1 cells stained with TCR . -SCBT QC
Date published: 2013-08-26
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