TCR alpha / beta Antibody (WT31)recommended for detection of the conformational epitope formed by T-cell receptor (TCR) for antigen and the CD3 epsilon chain of human origin by FCM

TCR α/β Antibody (WT31): sc-20078

TCR α/β Antibody (WT31) is rated 4.5 out of 5 by 2.
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Datasheets

    • TCR α/β Antibody (WT31) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against thymocytes of human origin
    • TCR alpha / beta Antibody (WT31) is recommended for detection of the conformational epitope formed by T-cell receptor (TCR) for antigen and the CD3 epsilon chain of human origin by FCM
    • TCR alpha / beta Antibody (WT31) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of TCR α/β (WT31): sc-20078.
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Does clone WT31 compete with OKT3?

Asked by: Castalja
Thank you for your question. Unfortunately we do not have any information regarding this monoclonal antibody competing with OKT3.
Answered by: Tech Service
Date published: 2019-07-08

Can TCR α/β (WT31): sc-20078 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Randy McDonald
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, TCR α/β (WT31): sc-20078 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Recommended for FCM Produces good data on Flow Cytometry of human samples
Date published: 2017-01-19
Rated 4 out of 5 by from Good FCM analysis of human peripheral blood Good FCM analysis of human peripheral blood leukocytes. -SCBT QC
Date published: 2014-03-20
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