Anti-Tau Antibody (A-12) recommended for detection of Tau of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-Tau Antibody (A-12): sc-166062

Anti-Tau Antibody (A-12) is rated 5.0 out of 5 by 2.
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Datasheets

    • Tau Antibody (A-12) is a mouse monoclonal IgG2b κ, cited in 2 publications, provided at 200 µg/ml
    • raised against amino acids 1-150 of Tau of human origin
    • recommended for detection of Tau of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • See Tau (Tau 46): sc-32274 for Tau antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG2b BP-HRP is the preferred secondary detection reagent for Tau Antibody (A-12) for WB and IHC(P) applications. This reagent is now offered in a bundle with Tau Antibody (A-12) (see ordering information below).
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I am using Tau (A-12): sc-166062 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Cweed
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Good Western Blot data of Tau expression Good Western Blot data of Tau expression in TE671 and SK-N-SH whole cell lysates. -SCBT QC
Date published: 2015-05-05
Rated 5 out of 5 by from Nice Western Blot data of Tau expression Nice Western Blot data of Tau expression in rat brain tissue extract. -SCBT QC
Date published: 2014-07-27
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