Syntaxin 8 Antibody (A-9)recommended for detection of Syntaxin 8 of human origin by WB, IP, IF and ELISA

Syntaxin 8 Antibody (A-9): sc-376521

Syntaxin 8 Antibody (A-9) is rated 4.7 out of 5 by 3.
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Datasheets

    • Syntaxin 8 Antibody (A-9) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-140 mapping at the N-terminus of Syntaxin 8 of human origin
    • Syntaxin 8 Antibody (A-9) is recommended for detection of Syntaxin 8 of human origin by WB, IP, IF and ELISA
    • Syntaxin 8 Antibody (A-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Syntaxin 8 (A-9): sc-376521.
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What blocking solution should I use with Syntaxin 8 (A-9): sc-376521 monoclonal antibody for Western blot?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-02-28
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Rated 5 out of 5 by from This antibody works great on Western Blot I used it to detect Syntaxin 8 from HEK293 cells and it gives a very good and specific band. The sensitivity is also very good. Highly recommend!
Date published: 2018-01-11
Rated 4 out of 5 by from not for hydra Tested in IHC in hydra vulgaris and did not react, as expected. Did not try in any other species
Date published: 2017-08-01
Rated 5 out of 5 by from Nice Western blot data of Syntaxin 8 expression Nice Western blot data of Syntaxin 8 expression in non-transfected and human Syntaxin 8 transfected 293 whole cell lysates. -SCBT QC
Date published: 2014-06-24
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