Syntaxin 18 Antibody (10)recommended for detection of Syntaxin 18 of mouse, rat, human and canine origin by WB, IP and IF

Syntaxin 18 Antibody (10): sc-293067

Syntaxin 18 Antibody (10) is rated 4.0 out of 5 by 2.
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Datasheets

    • Syntaxin 18 Antibody (10) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 220-331 of Syntaxin 18 of rat origin
    • recommended for detection of Syntaxin 18 of mouse, rat, human and canine origin by WB, IP and IF
    • available conjugated to agarose for IP; and to HRP for WB, IHC(P) and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Syntaxin 18 (10): sc-293067.
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What is the recommended fixation method for immunofluorescence staining with Syntaxin 18 (10): sc-293067 monoclonal antibody?

Asked by: Dr Ninau Qelp
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Strong signal in western blot analysis of Strong signal in western blot analysis of Syntaxin 18 expression in Jurkat whole cell lysate. -SCBT QC
Date published: 2015-04-18
Rated 4 out of 5 by from Robust immunofluorescence cytoplasmic staining Robust immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-01-08
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