Syntaxin 1 Antibody (SP6)recommended for detection of Syntaxin 1 of broad species origin by WB, IP and IF

Syntaxin 1 Antibody (SP6): sc-20036

Syntaxin 1 Antibody (SP6) is rated 4.5 out of 5 by 2.
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Datasheets

    • Syntaxin 1 Antibody (SP6) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against synaptic vesicle-containing fractions of immunoprecipitated human brain homogenate
    • recommended for detection of Syntaxin 1 of broad species origin by WB, IP and IF
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See Syntaxin 1 (HPC-1): sc-12736 for Syntaxin 1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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Could this be used in drosophila?

Asked by: Wangsc
Thank you for your question. This antibody has not been tested for Drosophila use, and is not recommended or warrantied for this use at this time. It is very possible it may work, but we do not have any testing data for this species. If you have any further questions, please contact Technical Service.
Answered by: Tech Service
Date published: 2019-09-06

What is the recommended starting dilution for Western blot for sc-20036: Syntaxin 1 (SP6) monoclonal antibody?

Asked by: jerojero
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from Worked really well in Western Blot against PC Worked really well in Western Blot against PC-12, SH-SY5Y and SK-N-SH whole cell lysates. -SCBT QC
Date published: 2015-06-15
Rated 4 out of 5 by from Great WB data obtained using mouse brain extract Great WB data obtained using mouse brain extract. -SCBT QC
Date published: 2013-10-08
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