Syntaxin 1 Antibody (HPC-1)recommended for detection of Syntaxin 1A and Syntaxin 1B of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Syntaxin 1 Antibody (HPC-1): sc-12736

Syntaxin 1 Antibody (HPC-1) is rated 4.5 out of 5 by 17.
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Datasheets

    • Syntaxin 1 Antibody (HPC-1) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against Syntaxin 1 of rat origin
    • Syntaxin 1 Antibody (HPC-1) is recommended for detection of Syntaxin 1A and Syntaxin 1B of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Syntaxin 1 Antibody (HPC-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Syntaxin 1 (HPC-1): sc-12736.
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Is Syntaxin 1 (HPC-1), sc-12736, available directly conjugated to HRP or fluorescent dyes?

Asked by: TinTin
Thank you for your question! Yes, sc-12736 is available conjugated to agarose (sc-12736 AC), HRP (sc-12736 HRP), phycoerythrin (sc-12736 PE), fluorescein (sc-12736 FITC), Alexa Fluor® 488 (sc-12736 AF488) and Alexa Fluor® 647 (sc-12736 AF647).
Answered by: Technical Support 2
Date published: 2017-01-13

What is the best secondary to use with this antibody in Western Blot?

Asked by: TinTin
Thank you for your question. We recommend using our Mouse IgG kappa Binding Protein HRP, sc-516102. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 7
Date published: 2017-01-10
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Rated 4 out of 5 by from Worked well by WB Worked well by Western Blot. SCBT Publication Review
Date published: 2019-11-14
Rated 1 out of 5 by from IF: No signals :( Unfortunately, did not work on cryosections (10µm) from human retinal organoids (IF 1:200/1:100/1:50).
Date published: 2019-07-23
Rated 4 out of 5 by from Good antibody This antibody worked very well with fixed frozen mouse tissue sections for IF
Date published: 2018-10-03
Rated 5 out of 5 by from amazing IHC I got an amazing fluorescent IHC on mouse retina frozen section using this antibody at 1:400. Should be able to use it at higher dilution.
Date published: 2018-05-25
Rated 5 out of 5 by from WB in NG108-15 cells I was able to detect a strong signal using WB performed in NG108-15 cells
Date published: 2018-05-17
Rated 5 out of 5 by from Great immunostaining I used this antibody on mouse retina frozen section (10 um) at 1/200 dilution in blocking solution (2% NHS/2 mg/mL BSA in 1x PBS/0.1% Triton X-100/0.05% NaN3) O/N at 4ºC.
Date published: 2017-11-06
Rated 5 out of 5 by from Successful application of this antibody in IF Successful application of this antibody in IF, using human tissues.
Date published: 2017-09-17
Rated 5 out of 5 by from Great antibody for WB It is a good antibody for western bolt, the background is clean and the band is specific.
Date published: 2017-02-10
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