Syntaxin 1 Antibody (4H256)recommended for detection of Syntaxin 1 of mouse, rat, human, Xenopus , bovine, porcine and feline origin by WB, IP and IF

Syntaxin 1 Antibody (4H256): sc-58297

Syntaxin 1 Antibody (4H256) is rated 4.0 out of 5 by 2.
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Datasheets

    • Syntaxin 1 Antibody (4H256) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against synaptic vesicle-containing fractions that were immunoprecipitated from human brain homogenates using anti-human Syntaxin 1 monoclonal antibodies
    • recommended for detection of Syntaxin 1 of mouse, rat, human, Xenopus , bovine, porcine and feline origin by WB, IP and IF; may cross-react with higher molecular weight proteins of unknown identity; non cross-reactive with a 35kDa protein in mouse liver extracts
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See Syntaxin 1 (HPC-1): sc-12736 for Syntaxin 1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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Hi, does this antibody bind to syntaxin 1a or 1b isoform? This is vital to know before purchase. Thanks.

Asked by: yfyf
Thank you for your question. This Syntaxin 1 antibody was raised against synaptic vesicle-containing fractions of immunoprecipitated human brain homogenate. The exact epitope has not been mapped, so we are unable to provide further information.
Answered by: Tech Service
Date published: 2019-10-30

What is the recommended secondary for Syntaxin 1 (4H256): sc-58297 monoclonal antibody?

Asked by: jenni
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support
Date published: 2017-03-07
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Rated 5 out of 5 by from Published immunocytochemical analysis using rMC Published immunocytochemical analysis using rMC-1 cell lysates. -SCBT Publication Review
Date published: 2015-02-14
Rated 3 out of 5 by from Positive result in western blot with background Positive result in western blot with background using murine brain tissue extract. -SCBT QC
Date published: 2014-04-28
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