syncytin Double Nickase Plasmid (h): sc-418235-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- syncytin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- syncytin Double Nickase Plasmid (h) and syncytin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ERVW-1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
syncytin Double Nickase Plasmid (h) | sc-418235-NIC | 20 µg | $410.00 | |||
syncytin Double Nickase Plasmid (h2) | sc-418235-NIC-2 | 20 µg | $410.00 |
ERVW-1 encodes syncytin, an envelope-derived fusogenic glycoprotein that promotes trophoblast cell–cell fusion and contributes to formation and maintenance of the placental syncytiotrophoblast. Syncytin-driven membrane fusion integrates with pathways regulating cell adhesion, cytoskeletal remodeling, and receptor-mediated signaling at the maternal–fetal interface, shaping differentiation and barrier function. Altered ERVW-1 expression or syncytin activity has been associated with dysregulated placentation and abnormal trophoblast biology, and has also been investigated in contexts where endogenous retroviral envelopes influence immune modulation and cellular stress responses. As a human-specific envelope protein, syncytin provides a model for studying how co-opted retroviral genes impact developmental programs and tissue homeostasis.
syncytin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ERVW-1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ERVW-1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ERVW-1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ERVW-1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.