Synaptotagmin IV Antibody (28-N)recommended for detection of Synaptotagmin IV of human origin by WB, IP, IF and ELISA

Synaptotagmin IV Antibody (28-N): sc-101302

Synaptotagmin IV Antibody (28-N) is rated 4.0 out of 5 by 3.
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Datasheets

    • Synaptotagmin IV Antibody (28-N) is a mouse monoclonal IgG3 (kappa light chain) provided at 100 µg/ml
    • raised against recombinant Synaptotagmin IV of human origin
    • recommended for detection of Synaptotagmin IV of human origin by WB, IP, IF and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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I am using Synaptotagmin IV (28-N): sc-101302 for immunofluorescence, what dilution of this antibody should I use?

Asked by: Trav11
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Great antibody for WB Great antibody for WB, works in A-431 whole cell lysate. -SCBT QC
Date published: 2015-07-06
Rated 4 out of 5 by from Performs well in Western Performs well in Western, good signal with mouse Synaptotagmin IV transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-06-09
Rated 4 out of 5 by from Produced great immunofluorescence membrane Produced great immunofluorescence membrane and cytoplasmic staining in paraformaldehyde-fixed A-431 cells. -SCBT QC
Date published: 2015-05-13
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