Anti-SVCT1 Antibody (H-11) recommended for detection of SVCT1 of human origin by WB, IP, IF, IHC(P) and ELISA

Anti-SVCT1 Antibody (H-11): sc-376090

Anti-SVCT1 Antibody (H-11) is rated 5.0 out of 5 by 2.
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Datasheets

    • Anti-SVCT1 Antibody (H-11) is a mouse monoclonal IgG1 κ SVCT1 antibody, cited in 3 publications, provided at 200 µg/ml
    • raised against amino acids 521-598 mapping at the C-terminus of SVCT1 of human origin
    • SVCT1 Antibody (H-11) is recommended for detection of SVCT1 of human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-SVCT1 Antibody (H-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of SVCT1 (H-11): sc-376090.
    • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for SVCT1 Antibody (H-11) for WB and IHC(P) applications. These reagents are now offered in bundles with SVCT1 Antibody (H-11) (see ordering information below).
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Has this antibody been used for flowcytometry?

Asked by: hkazemi
Thank you for your question. SVCT1 Antibody (H-11): sc-376090 is recommended for detection of SVCT1 of human origin by WB, IP, IF, IHC(P) and ELISA. It has not been tested in flow cytometry. Please get in touch with our technical service to arrange for a free sample of this antibody to test it in your experimental setup.
Answered by: Technical Support Europe
Date published: 2019-04-30

I am using SVCT1 (H-11): sc-376090 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: TinTin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24

Hi, Does the antibody binding to the extracellular or intracellular domains or both?

Asked by: Oslem TCD
Thank you for your question. This antibody, sc-376090 SVCT1 (H-11), was raised against amino acids 521-598 mapping within a cytoplasmic domain near the C-terminal of human SVCT1 (accession#Q9UHI7). If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-01-17

Can I use this antibodies in chicken?

Asked by: dawn
Thank you for your question. This antibody has not been tested in chicken samples and I can not find any sequence for SVCT1 in chicken, so I can not do a sequence homology check for you. The exact immunogen sequence is considered proprietary. However, I can provide you with a 50 amino acid range of where the immunogen is located. SVCT1 (H-11): sc-376090 was raised against a sequence between amino acids 548-598 of SVCT1 of human origin (accession# Q9UHI7). If you have any further questions, please contact our Asia Technical Service team. You can reach them by phone at (86 21) 6093-6350, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2016-11-14

What is the volume or weight of one unit, you are only stating 200 ug/ml, so a concentration. Also, would you be able to supply larger amounts, say 1 milligram? Please quote.

Asked by: Eva CdM
Thank you for your inquiry. The total volume provided is 1 ml. For quotes for larger quantities, please contact our Special Orders department at [email protected]
Answered by: Technical Service
Date published: 2016-11-03
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Rated 5 out of 5 by from Superb immunoperoxidase apical membrane and Superb immunoperoxidase apical membrane and cytoplasmic staining in formalin fixed, paraffin-embedded human kidney tissue. -SCBT QC
Date published: 2015-03-28
Rated 5 out of 5 by from Good Western Blot data of SVCT1 expression in SH Good Western Blot data of SVCT1 expression in SH-SY5Y whole cell lysate. -SCBT QC
Date published: 2014-01-13
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