SUGT1 Antibody (B-3)recommended for detection of SUGT1 of mouse, rat and human origin by WB, IP, IF and ELISA

SUGT1 Antibody (B-3): sc-398639

SUGT1 Antibody (B-3) is rated 5.0 out of 5 by 2.
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Datasheets

    • SUGT1 Antibody (B-3) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 141-365 mapping at the C-terminus of SUGT1 of human origin
    • recommended for detection of SUGT1 of mouse, rat and human origin by WB, IP, IF and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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What is the recommended fixation method for immunofluorescence staining with SUGT1 (B-3): sc-398639 monoclonal antibody?

Asked by: Randy McDonald
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Produced beautiful immunofluorescence cytoplasmic Produced beautiful immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-05-06
Rated 5 out of 5 by from Produced positive Western Blot data of SUGT1 Produced positive Western Blot data of SUGT1 expression in A-431 whole cell lysate. -SCBT QC
Date published: 2014-10-21
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