Sudan III CAS: 85-86-9
MF: C22H16N4O
MW: 352.39
A lysochrome (fat soluble dye) predominantly used for staining triglycerides in animal tissues (frozen sections).

Sudan III (CAS 85-86-9)

Sudan III | CAS 85-86-9 is rated 5.0 out of 5 by 1.
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Alternate Names: Solvent Red 23; CI 26100
Application: Sudan III is a lysochrome (fat soluble dye) predominantly used for staining triglycerides in animal tissues (frozen sections)
CAS Number: 85-86-9
Molecular Weight: 352.39
Molecular Formula: C22H16N4O
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
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Sudan III is predominantly used for staining triglycerides in animal tissues (frozen sections). Sudan dyes are a group of lipid soluble solvent dyes often called lysochromes, in structural classification they are diazo dyes. With the use of certain solvents, may also be used to stain some protein bound lipids in paraffin sections. Sudan I (sc-215922), Sudan III (sc-203761), and Sudan IV (sc-203762) have been classified as category 3 carcinogens by the International Agency for Research on Cancer.


References

1. Kodama, H., et al. 1977. J. Dermatol. 4: 41-48. PMID: 15461324
2. Masamune, O., et al. 1977. Tohoku J. Exp. Med. 122: 397-402. PMID: 72437
3. Yoshimura, S., et al. 1980. Acta Cytol. 24: 44-48. PMID: 6153489
4. Refat, N.A., et al. 2008. J. Biochem. Mol. Toxicol. 22: 77-84. PMID: 18418879

Usage :
• A saturated solution in isopropanol is prepared for use as a fat stain in animal tissue. Counterstain for 5 minutes in acid alum hematoxylin (approx. 0.1%). Fats will appear yellow orange in color and the nuclei will appear blue. The cytoplasm will appear green.

• Since lysochromes are soluble in non polar solvents, including xylene and toluene, and also in ethanol, these fluids must not be put on to sections after fats have been stained or the dye will be dissolved out; therefore resinous mounting media are not recommended. We recommend mounting sections with Crystal Mounting Medium; sc-24943.
Appearance :
Powder
Physical State :
Solid
Solubility :
Soluble in ether, acetone, chloroform, benzene, ethanol (slightly soluble), DMSO, Hot glacial acetic acid (Slightly), hot glycerol, and toluene. Insoluble in water, and Lye.
Storage :
Store at room temperature
Melting Point :
199° C (dec.)
Boiling Point :
~584.6° C at 760 mmHg (Predicted)
Density :
~1.2 g/cm3 (Predicted)
Refractive Index :
n20D 1.66 (Predicted)
pK Values :
pKa: 7.51
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
RTECS :
QK4250000
PubChem CID :
Merck Index :
14: 8884
MDL Number :
MFCD00003905
EC Number :
201-638-4
Beilstein Registry :
931185
SMILES :
C1=CC=C(C=C1)N=NC2=CC=C(C=C2)N/N=C/3\\C(=O)C=CC4=CC=CC=C43

Download SDS (MSDS)

Certificate of Analysis

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Sudan III (CAS 85-86-9)  Product Citations

See how others have used Sudan III (CAS 85-86-9). Click on the entry to view the PubMed entry .

Citations 1 to 2 of 2 total

PMID: # 12853974  Steiling H, et al. 2003. Oncogene. 22: 4380-4388.

PMID: # 11788470  Namiki, M. et al. 2002. Arterioscler Thromb Vasc Biol. 22: 115-120.

Citations 1 to 2 of 2 total

How to get Sudan 3 dye stain out of wood without damaging the wood.

Asked by: QueenoftheLab
Thank you for your unusual, yet interesting question! Unfortunately, we are unable to provide this information. We did research your question, and were unable to find any literature on it.
Answered by: Technical Support
Date published: 2019-04-26
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Rated 5 out of 5 by from Namiki et al Namiki et al. (PubMed ID 11788470) used the lysochrome, sudan III, to visualize lesions caused by the infiltration of RAM-11 into arterial walls. -SCBT Publication Review
Date published: 2015-06-18
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