Sucrase-Isomaltase Antibody (C-8)recommended for detection of precursor and mature isomaltase isoform of Sucrase-Isomaltase of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Sucrase-Isomaltase Antibody (C-8): sc-393470

Sucrase-Isomaltase Antibody (C-8) is rated 5.0 out of 5 by 2.
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Datasheets

    • Sucrase-Isomaltase Antibody (C-8) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 136-167 near the N-terminus of Sucrase-Isomaltase of rat origin
    • Sucrase-Isomaltase Antibody (C-8) is recommended for detection of precursor and mature isomaltase isoform of Sucrase-Isomaltase of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Sucrase-Isomaltase Antibody (C-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-393470 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Sucrase-Isomaltase (C-8): sc-393470.
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I am planning on using this Sucrase-Isomaltase (C-8): sc-393470 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: TinTin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6.0) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining.
Answered by: Tech Service
Date published: 2017-02-01
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Rated 5 out of 5 by from Good Western Blot We used this antibody in a western blot experiment for rat tissue and got great results
Date published: 2016-12-27
Rated 5 out of 5 by from Satisfactory Western blot data of Sucrase Satisfactory Western blot data of Sucrase-Isomaltase expression in rat small intestine tissue extract. -SCBT QC
Date published: 2014-04-15
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