STEAP Antibody (B-4) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
raised against amino acids 1-105 of STEAP of human origin
STEAP Antibody (B-4) is recommended for detection of STEAP of human origin by WB, IP, IF and ELISA
STEAP Antibody (B-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of STEAP (B-4): sc-271872.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Six-transmembrane epithelial antigen of the prostate (STEAP) is structurally similar to a channel or transport protein. STEAP protein contains six potential membrane-spanning regions with hydrophilic amino- and carboxyl-terminal domains. STEAP protein is present in human prostate tissue with elevated levels in cancer cell lines, including prostate, bladder, colon, ovarian, and Ewing sarcoma. Cell-cell junctions of the secretory epithelium show concentrated levels of STEAP protein. Mouse STEAP is 80% homologous to human STEAP at both the nucleotide and amino acid levels. The human STEAP gene maps to chromosome 7q21 and encodes a 339 amino acid protein.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Is this antibody(sc-271872;STEAP antibody) suitable for immunostaining?
If so, what is the recommended starting dilution?
Asked by: Anonymous
Thank you for your question. This primary antibody is recommended and warrantied for IF (immunofluorescent staining of fresh or frozen tissues or cells fixed in methanol or acetone), however we are still gathering data to show on our website. Check back for updates! We recommend a starting dilution of 1:50 (range 1:50-1:500).
Answered by: Technical Support
Date published: 2019-04-22
What is the recommended starting dilution for Western blot for sc-271872: STEAP (B-4) monoclonal antibody?
Asked by: Professor Griffin
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol:
Rated 5 out of
Randy McDonald from
Nice antibody for western blotWe used this antibody for expression detection of STEAP by western blot. We were able to get nice bands around the correct molecular weight
Date published: 2017-02-03
Rated 5 out of
Produced nice Western blot data of STEAPProduced nice Western blot data of STEAP expression in LNCaP whole cell lysate. -SCBT QC
To place an order using RMB or to ship to mainland China, please visit www.scbio.cn
Create a new account
Email address already exists, please enter a new valid email address.Hide
USE YOUR SOCIAL NETWORK
Create an account quickly and easily with your preferred social network account. You won't have to remember an extra name and password.
Creating an account with us makes your shopping experience much easier and faster. You can save favorites, save cart, check order status and speed through checkout with saved addresses, payment methods and more.