STEAP Antibody (B-4)recommended for detection of STEAP of human origin by WB, IP, IF and ELISA

STEAP Antibody (B-4): sc-271872

STEAP Antibody (B-4) is rated 5.0 out of 5 by 2.
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Datasheets

    • STEAP Antibody (B-4) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-105 of STEAP of human origin
    • STEAP Antibody (B-4) is recommended for detection of STEAP of human origin by WB, IP, IF and ELISA
    • STEAP Antibody (B-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of STEAP (B-4): sc-271872.
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Is this antibody(sc-271872;STEAP antibody) suitable for immunostaining? If so, what is the recommended starting dilution?

Asked by: Anonymous
Thank you for your question. This primary antibody is recommended and warrantied for IF (immunofluorescent staining of fresh or frozen tissues or cells fixed in methanol or acetone), however we are still gathering data to show on our website. Check back for updates! We recommend a starting dilution of 1:50 (range 1:50-1:500).
Answered by: Technical Support
Date published: 2019-04-22

What is the recommended starting dilution for Western blot for sc-271872: STEAP (B-4) monoclonal antibody?

Asked by: Professor Griffin
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from Nice antibody for western blot We used this antibody for expression detection of STEAP by western blot. We were able to get nice bands around the correct molecular weight
Date published: 2017-02-03
Rated 5 out of 5 by from Produced nice Western blot data of STEAP Produced nice Western blot data of STEAP expression in LNCaP whole cell lysate. -SCBT QC
Date published: 2013-07-01
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