StaurosporineAn antifungal inhibitor of many different enzymes

Staurosporine (CAS 62996-74-1)

Staurosporine | CAS 62996-74-1 is rated 5.0 out of 5 by 4.
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Synonym: Antibiotic AM-2282
Application: An antifungal inhibitor of many different enzymes
CAS Number: 62996-74-1
Purity: >99%
Molecular Weight: 466.53
Molecular Formula: C28H26N4O3
* Refer to Certificate of Analysis for lot specific data (including water content).
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Staurosporine, a microbial alkaloid with antifungal activity, has been shown to inhibit a variety of kinases including PKA (Ki=7.0 nM), PKG (Ki=8.5 nM), MYLK (MLCK, Ki=1.3 nM), PKC (Ki=0.7 nM), CaMK (IC50=20 nM), CAMKII (Ki=20nM), tyrosine kinases (IC50=70 nM) and phosphorylase kinase (IC50=0.5 nM). Inhibition is via interaction with the ATP binding site. It induces association of purified PKC with inside-out vesicles from erythrocyte membranes and augments PMA-induced ornithine decarboxylase. Staurosporine activates a bcl-2-regulated apoptosis pathway. Further, there is evidence that it inhibits c-Src, Cdc2, Cdk2, Cdk4, Cdk5, cGKI, cyclin A, cyclin B, cyclin D, Fgr, GSK-3 beta, Lyn, p35, Pim-3, PKC alpha, Syk and Topo II.


References

1. Tamaoki, T., et al. 1986. Biochem. Biophys. Res. Commun. 135: 397-402. PMID: 3457562
2. Wolf, M., et al. 1988. Biochem. Biophys. Res. Commun. 154: 1273-1279. PMID: 3408497
3. Matsumoto, H., et al. 1989. Biochem. Biophys. Res. Commun. 158: 105-109. PMID: 2912441
4. Fallon, R.J. 1990. Biochem. Biophys. Res. Commun. 170: 1191-1196. PMID: 2167672
5. Elliott, L.H., et al. 1990. Biochem. Biophys. Res. Commun. 171: 148-154. PMID: 2393389
6. Badwey, J.A., et al. 1991. Biochem. Biophys. Res. Commun. 178: 423-429. PMID: 1859401
7. Yanagihara, N., et al. 1991. J. Neurochem. 56: 294-298. PMID: 1846174

Physical State :
Solid
Derived From :
Streptomyces sp. MST-AS5345
Solubility :
Soluble in DMSO (25 mg/ml), DMF (25 mg/ml), ethyl acetate, hot acetone, and ethanol. Slightly soluble in chloroform, and methanol, Insoluble in water.
Storage :
Store at -20° C
Melting Point :
240-243° C
Boiling Point :
~677.5° C at 760 mmHg (Predicted)
Density :
~1.6 g/cm3 (Predicted)
Refractive Index :
n20D 1.81
IC50 :
Chk1: IC50 = <0.5 nM (human); Akt3: IC50 = 0.64 nM (human); phosphorylase kinase: IC50 = 0.5 nM; Tyrosine-protein kinase SRC: IC50 = 1.0 nM (human); AMPK: IC50 = <1.0 nM (human)
Ki Data :
Polo-Like Kinase 4: Ki= 2.6 nM (human); Tyrosine-protein kinase ITK: Ki= 2.2 nM (human); MLCK: Ki= 1.3 nM; PKC: Ki= 0.7 nM; CDK1: Ki= 1.0 nM (human)
pK Values :
pKb: 8.97
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
RTECS :
KD5084000
PubChem CID :
44259
Merck Index :
14: 8802
MDL Number :
MFCD00077402
SMILES :
C[C@@]12[C@@H]([C@@H](C[C@@H](O1)N3C4=CC=CC=C4C5=C6C(=C7C8=CC=CC=C8N2C7=C53)CNC6=O)NC)OC

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Certificate of Analysis

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Staurosporine  Product Citations

See how others have used Staurosporine. Click on the entry to view the PubMed entry .

Citations 1 to 10 of 50 total

PMID: # 30967464  Giebel, AM.|Hu, S.|Rajaram, K.|Finethy, R.|Toh, E.|Brothwell, JA.|Morrison, SG.|Suchland, RJ.|Stein, BD.|Coers, J.|Morrison, RP.|Nelson, DE.| et al. 2019. MBio. 10:

PMID: # 31325684  Ribeiro, V. et al. 2019. Phytomedicine. 63: 153017.

PMID: # 30139286  Chen, X.|Zha, GF.|Wang, JQ.|Liu, XH.| et al. 2018. J Enzyme Inhib Med Chem. 33: 1266-1270.

PMID: # 30006243  Luczak, MW.|Zhitkovich, A.| et al. 2018. Toxicol. Appl. Pharmacol. 355: 238-246.

PMID: # 28234980  Faria, M. et al. 2017. PLoS ONE. 12: e0172689.

PMID: # 28343940  Magiera, K. et al. 2017. Cell Chem Biol. 24: 458-470.e18.

PMID: # 28837145  Cheng, X.|Shi, JB.|Liu, H.|Chen, LZ.|Wang, Y.|Tang, WJ.|Liu, XH.| et al. 2017. Cell Death Dis. 8: e3016.

PMID: # 28300624  da Silva, DC. et al. 2017. J. Steroid Biochem. Mol. Biol. 171: 178-186.

PMID: # 26900656  Wang, Y. et al. 2016. European journal of medicinal chemistry. 112: 231-51.

PMID: # 27037191  Gaidt, MM. et al. 2016. Immunity. 44: 833-46.

Citations 1 to 10 of 50 total

Once dissolved in DMSO, how long is this stable for?

Asked by: 13punMA
Thank you for asking about our Staurosporine (CAS# 62996-74-1). The chemical is stable for a few months when stored at -20&ordm;C. Stock solutions can be stable for up to 6 months when stored at -70&ordm;C.
Answered by: Chemical Support 8
Date published: 2017-03-01

What is the chemical grade for this product, sc-3510?

Asked by: SCM4
Thank you for your question. We do not offer a specific grade for Staurosporine (CAS 62996-74-1): sc-3510, however the purity is &ge;99%.
Answered by: Technical Support
Date published: 2016-12-14

After resuspending using DMSO, how should the solution be stored?

Asked by: chemicalsmg
Thank you for your question. If necessary to store Staurosporine (CAS 62996-74-1) as resuspension, aliquot out and store at -20&ordm;C. Avoid repeated freeze/thaw cycles.
Answered by: Technical Support
Date published: 2016-12-14
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Rated 5 out of 5 by from Staurosporine, 100 ug Staurosporine was purchased from Santa Cruz Biotechnology on May 8, 2017. The compound was reconstituted in DMSO. Human AML cells line MOLM13 was treated in the growth medium containing 100 nM and 500 nM of staurosporine respectively at 37C for 24h. Western blotting was performed for characterization of caspase 3. Conclusion: Staurosporine effectively stimulates the activation process of caspase 3 in MOLM13 cells.
Date published: 2017-06-13
Rated 5 out of 5 by from Satisfied with product Satisfied with product.
Date published: 2015-10-07
Rated 5 out of 5 by from No comment No comment.
Date published: 2015-04-13
Rated 5 out of 5 by from Broad enzymatic inhibitor and also inhibitor Broad enzymatic inhibitor and also inhibitor of a variety of kinases. In order to inhibit, staurosporine interferes with the ATP binding site. -SCBT QC
Date published: 2015-02-11
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